SERBP1 antibody (ab57285) used in immunofluorescence at 10ug/ml on HeLa cells.
Western blot - SERBP1 antibody (ab57285)
Predicted band size : 45 kDa SERBP1 antibody (ab57285) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Flow Cytometry - Anti-SERBP1 antibody (ab57285)
Overlay histogram showing HEK293 cells stained with ab57285 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57285, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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