製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to SEPT12
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-358 of Human FLJ25410

  • ポジティブ・コントロール
    • Hela cells, transfected 293T cell line.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis.
  • 組織特異性
    Widely expressed.
  • 配列類似性
    Belongs to the septin family.
  • 細胞内局在
    Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. At interphase, forms a filamentous structure in the cytoplasm. During anaphase, translocates to the central spindle region and to the midbody during cytokinesis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • SEP12_HUMAN antibody
    • SEPT12 antibody
    • Septin-12 antibody
    • Septin12 antibody

Anti-SEPT12 antibody 画像

  • Immunofluorescent analysis of FLJ25410 expression in HeLa cells using 10 µg/ml ab66960.
  • All lanes : Anti-SEPT12 antibody (ab66960) at 1/1000 dilution

    Lane 1 : FLJ25410 transfected 293T cell lysate
    Lane 2 : untransfected 293T cell lysate

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

Anti-SEPT12 antibody (ab66960) 使用論文

ab66960 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab66960.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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