Anti-Separase (phospho S748) 抗体 (ab59992)

製品の概要

  • 製品名Anti-Separase (phospho S748) antibody
    Separase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Separase (phospho S748)
  • アプリケーション適用あり: ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phosphopeptide corresponding to amino acid residues surrounding S748 of human Separase conjugated to KLH

  • ポジティブ・コントロール
    • Human hepatocarcinoma tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)ab59992 is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
  • 配列類似性Belongs to the peptidase C50 family.
  • 翻訳後修飾Autocleaves. This function, which is not essential for its protease activity, is unknown.
    Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Caspase like protein ESPL1 antibody
    • Caspase-like protein ESPL1 antibody
    • ESP 1 antibody
    • ESP1 antibody
    • ESPL 1 antibody
    • Espl1 antibody
    • ESPL1_HUMAN antibody
    • Extra spindle poles like 1 antibody
    • Extra spindle poles like 1 protein antibody
    • Extra spindle poles-like 1 protein antibody
    • FLJ46492 antibody
    • KIAA0165 antibody
    • Separase antibody
    • Separin antibody
    • Similar to fission yeast cut1and gene antibody
    • SSE antibody
    see all

Anti-Separase (phospho S748) antibody 画像

  • ab59992 at 1/50-1/100 dilution staining Separase in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. The primary antibody was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Anti-Separase (phospho S748) antibody (ab59992) 使用論文

ab59992 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"