製品の概要

  • 製品名Anti-Separase antibody
    Separase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Separase
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between amino acids 1425-1475 of human Separase (NP_036423.3).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 5-10 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 233 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
    • 配列類似性Belongs to the peptidase C50 family.
    • 翻訳後修飾Autocleaves. This function, which is not essential for its protease activity, is unknown.
      Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
    • 細胞内局在Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Caspase like protein ESPL1 antibody
      • Caspase-like protein ESPL1 antibody
      • ESP 1 antibody
      • ESP1 antibody
      • ESPL 1 antibody
      • Espl1 antibody
      • ESPL1_HUMAN antibody
      • Extra spindle poles like 1 antibody
      • Extra spindle poles like 1 protein antibody
      • Extra spindle poles-like 1 protein antibody
      • FLJ46492 antibody
      • KIAA0165 antibody
      • Separase antibody
      • Separin antibody
      • Similar to fission yeast cut1and gene antibody
      • SSE antibody
      see all

    Anti-Separase antibody 画像

    • All lanes : Anti-Separase antibody (ab85685) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 233 kDa
      Observed band size : ~233 kDa (why is the actual band size different from the predicted?)
      Additional bands at : ~175 kDa,~38 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Separase was immunoprecipitated using ab85685 at 10 µg/mg whole cell lysate from HeLa cells.
      20% of IP was loaded per lane, and probed with ab85685 at 1 µg/ml (lane 1) or with a control IgG (lane 2).
      Detection: chemiluminescence with exposure time of 10 seconds.
      Note: The more rapidly migrating band (MW ~175kDa) is believed to represent an additional form of Separase owing to recognition by multiple antibodies each of which was directed toward distinctly different epitopes on Separase.

    Anti-Separase antibody (ab85685) 使用論文

    ab85685 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab85685.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"