製品の概要

  • 製品名Anti-Separase antibody
    Separase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Separase
  • 特異性ab52158 detects endogenous levels of total SEPARASE protein.
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic non-phosphopeptide derived from human Separase around the phosphorylation site of serine 801 (N-S-S-P-V).

  • ポジティブ・コントロール
    • human colon carcinoma, extracts from A431 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately >175 kDa (predicted molecular weight: 233 kDa).
ELISA 1/40000.

ターゲット情報

  • 機能Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
  • 配列類似性Belongs to the peptidase C50 family.
  • 翻訳後修飾Autocleaves. This function, which is not essential for its protease activity, is unknown.
    Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Caspase like protein ESPL1 antibody
    • Caspase-like protein ESPL1 antibody
    • ESP 1 antibody
    • ESP1 antibody
    • ESPL 1 antibody
    • Espl1 antibody
    • ESPL1_HUMAN antibody
    • Extra spindle poles like 1 antibody
    • Extra spindle poles like 1 protein antibody
    • Extra spindle poles-like 1 protein antibody
    • FLJ46492 antibody
    • KIAA0165 antibody
    • Separase antibody
    • Separin antibody
    • Similar to fission yeast cut1and gene antibody
    • SSE antibody
    see all

Anti-Separase antibody 画像

  • All lanes : Anti-Separase antibody (ab52158) at 1/500 dilution

    Lane 1 : extracts from A431 cells
    Lane 2 : extracts from A431 cells, with immunizing peptide


    Predicted band size : 233 kDa
    Observed band size : 233 kDa
  • Immunohistochemical analysis of separase expression in paraffin embedded human colon carcinoma tissue using ab52158 (1:50 dilution). Left: sample untreated. Right: sample treated with immunizing peptide (negative control).

Anti-Separase antibody (ab52158) 使用論文

ab52158 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab52158.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"