リコンビナント
RabMAb

Anti-SEP15 抗体 [NCIR128A] (ab124840)

製品の概要

  • 製品名
    Anti-SEP15 antibody [NCIR128A]
    SEP15 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [NCIR128A] to SEP15
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse SEP15.

  • ポジティブ・コントロール
    • Rat liver, fetal liver, human testis, mouse testis, rat testis and LnCaP lysates.
  • 特記事項

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    NCIR128A
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.

ターゲット情報

画像

  • Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution (purified) + Human testis lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 18 kDa
    Observed band size: 15 kDa (why is the actual band size different from the predicted?)



    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution (unpurified)

    Lane 1 : Rat liver lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : LnCaP liver lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 18 kDa

プロトコール

参考文献

This product has been referenced in:
  • Ekoue DN  et al. Correlations of SELENOF and SELENOP genotypes with serum selenium levels and prostate cancer. Prostate 78:279-288 (2018). Read more (PubMed: 29314169) »
  • Tsuji PA  et al. The 15kDa selenoprotein and thioredoxin reductase 1 promote colon cancer by different pathways. PLoS One 10:e0124487 (2015). Human . Read more (PubMed: 25886253) »

See all 3 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole body)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
whole body
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Apr 23 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録