製品の概要

  • 製品名Anti-SEP15 antibody
    SEP15 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SEP15
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    A synthetic peptide within residues

    VRSDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEF

    corresponding to amino acids 109-158 of Human SEP15 (NP_004252)

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-SEP15 antibody 画像

  • Anti-SEP15 antibody (ab80985) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 15 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.

Anti-SEP15 antibody (ab80985) 使用論文

This product has been referenced in:
  • Miniard AC  et al. Nucleolin binds to a subset of selenoprotein mRNAs and regulates their expression. Nucleic Acids Res 38:4807-20 (2010). Read more (PubMed: 20385601) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (primary B cells, 3T3 cells)
Loading amount 40 µg
Specification primary B cells, 3T3 cells
Gel Running Conditions Reduced Denaturing (Gradient gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Feb 10 2012

Thank you for contacting us.

We have not validated this antibody for use on mouse, and therefore do not batch test it on mouse samples. If you would like to test the recent batch on a positive control from human samples, we would be happy to...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"