製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to SENP6
  • 特異性
    ab77619 is expected to recognise both reported isoforms (NP_001093879.1; NP_056386.2).
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    C-KPKYEPNPHYHEN

    , corresponding to internal sequence amino acids 778-790 of Human SENP6 (NP_056386.2).

  • ポジティブ・コントロール
    • HeLa cell nuclear lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    Peptide ELISA: Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison.
    • 組織特異性
      Highly expressed in reproductive organs, such as testis, ovary and prostate.
    • パスウェイ
      Protein modification; protein sumoylation.
    • 配列類似性
      Belongs to the peptidase C48 family.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 2810017C20Rik antibody
      • FLJ11355 antibody
      • FLJ11887 antibody
      • KIAA0389 antibody
      • KIAA0797 antibody
      • SENP 6 antibody
      • Senp6 antibody
      • SENP6_HUMAN antibody
      • Sentrin specific protease 6 antibody
      • Sentrin-specific protease 6 antibody
      • Sentrin/SUMO specific protease SENP6 antibody
      • Sentrin/SUMO-specific protease SENP6 antibody
      • SSP 1 antibody
      • SSP1 antibody
      • SUMO 1 specific protease 1 antibody
      • SUMO 1 specific protease antibody
      • SUMO-1-specific protease 1 antibody
      • SUMO1 specific protease 1 antibody
      • SUMO1 specific protease antibody
      • SUMO1/sentrin specific peptidase 6 antibody
      • SUSP 1 antibody
      • SUSP1 antibody
      see all

    画像

    プロトコール

    参考文献

    ab77619 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Recombinant protein (E.coli produced recombinant SENP6)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    0.2 µg
    Specification
    E.coli produced recombinant SENP6
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 07 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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