製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SENP5
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide from an internal region of Human SENP5

  • ポジティブ・コントロール
    • Human lung carcinoma tissue. COS-7 cell extracts

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

ターゲット情報

  • 機能
    Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division.
  • 配列類似性
    Belongs to the peptidase C48 family.
  • 細胞内局在
    Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FKSG45 antibody
    • FLJ42398 antibody
    • MGC27076 antibody
    • Protease FKSG45 antibody
    • SENP5 antibody
    • SENP5_HUMAN antibody
    • Sentrin specific protease SENP5 antibody
    • Sentrin-specific protease 5 antibody
    • Sentrin/SUMO specific protease SENP5 antibody
    • Sentrin/SUMO-specific protease SENP5 antibody
    • SUMO specific protease SENP5 antibody
    • SUMO1/sentrin specific peptidase 5 antibody
    • SUMO1/sentrin specific protease 5 antibody
    see all

画像

  • Immunohistochemical analysis of paraffin embedded human lung carcinoma using ab58420 at 1/50 dilution, in the absence or presence of the immunizing peptide.
  • All lanes : Anti-SENP5 antibody (ab58420) at 1/500 dilution

    Lane 1 : Cos 7 cell extract
    Lane 2 : Cos 7 cell extract with immunizing peptide


    Predicted band size : 87 kDa
    Observed band size : 87 kDa

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton 5 minutes
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 20 2017

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 03 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human osteosarcoma cells)
Loading amount
20 µg
Specification
human osteosarcoma cells
Treatment
siRNA transfected with control and two senp5 siRNA
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Sonia Rocha

Verified customer

投稿 Jun 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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