製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SENP2
  • アプリケーション
    適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from an internal region of Human SENP2

  • ポジティブ・コントロール
    • MDA-MB-435 cell extracts

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
ELISA 1/20000.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway.
  • 配列類似性
    Belongs to the peptidase C48 family.
  • ドメイン
    The N-terminus is necessary and sufficient for nuclear envelope targeting.
  • 翻訳後修飾
    Polyubiquitinated; which leads to proteasomal degradation.
  • 細胞内局在
    Nucleus > nuclear pore complex. Nucleus membrane. Cytoplasm. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Axam2 antibody
    • DKFZp762A2316 antibody
    • KIAA1331 antibody
    • Senp2 antibody
    • SENP2_HUMAN antibody
    • Sentrin specific protease 2 antibody
    • Sentrin-specific protease 2 antibody
    • Sentrin/SUMO specific protease SENP2 antibody
    • Sentrin/SUMO-specific protease SENP2 antibody
    • SMT3 specific isopeptidase 2 antibody
    • SMT3-specific isopeptidase 2 antibody
    • Smt3ip2 antibody
    • SUMO1/sentrin/SMT3 specific peptidase 2 antibody
    see all

画像

  • All lanes : Anti-SENP2 antibody (ab58418) at 1/500 dilution

    Lane 1 : MDA-MB-435 cell extract
    Lane 2 : MDA-MB-435 cell extract with immunizing peptide


    Predicted band size : 68 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab58418 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58418, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Snow JW  et al. Sumoylation Regulates Interaction of FOG1 with C-terminal-binding Protein (CTBP). J Biol Chem 285:28064-75 (2010). WB ; Human . Read more (PubMed: 20587419) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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