製品の概要

  • 製品名Anti-SENP1 antibody
    SENP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SENP1
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide derived from the N-terminus of Human SENP1

  • ポジティブ・コントロール
    • Human breast carcinoma tissue. Jurkat cells extract

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

ターゲット情報

  • 機能Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1, which decreases its transcriptional repression activity.
  • 組織特異性Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.
  • 配列類似性Belongs to the peptidase C48 family.
  • 細胞内局在Nucleus. Cytoplasm. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • SENP 1 antibody
    • SENP1 antibody
    • SENP1_HUMAN antibody
    • Sentrin specific protease 1 antibody
    • Sentrin-specific protease 1 antibody
    • Sentrin/SUMO specific protease 1 antibody
    • Sentrin/SUMO specific protease antibody
    • Sentrin/SUMO specific protease SENP 1 antibody
    • Sentrin/SUMO specific protease SENP1 antibody
    • Sentrin/SUMO-specific protease SENP1 antibody
    • SUMO1/sentrin specific peptidase 1 antibody
    • SUMO1/sentrin specific protease 1 antibody
    • SuPr 2 antibody
    • SuPr2 antibody
    see all

Anti-SENP1 antibody 画像

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab58417 at 1/50 dilution. Samples are treated -/+ blocking peptide.
  • All lanes : Anti-SENP1 antibody (ab58417) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunizing peptide


    Predicted band size : 73 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

Anti-SENP1 antibody (ab58417) 使用論文

This product has been referenced in:
  • Huang W  et al. Triptolide Inhibits the Proliferation of Prostate Cancer Cells and Down-Regulates SUMO-Specific Protease 1 Expression. PLoS One 7:e37693 (2012). WB . Read more (PubMed: 22666381) »
  • Snow JW  et al. Sumoylation Regulates Interaction of FOG1 with C-terminal-binding Protein (CTBP). J Biol Chem 285:28064-75 (2010). WB ; Human . Read more (PubMed: 20587419) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Mouse Tissue lysate - whole (brain)
Specification brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jan 26 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

投稿 Feb 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"