製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SENP1
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide:

    CKRKEFDTNGWQLFSKK

    , corresponding to amino acids 575-590 of Human SENP 1.

  • ポジティブ・コントロール
    • HL60 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    Western blotting: 1/100 - 1/500.
    ELISA: 1/1000.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    Expected molecular weight: 73.351 kDa (from SwissProt Q9P0U3).
  • ターゲット情報

    • 機能
      Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1, which decreases its transcriptional repression activity.
    • 組織特異性
      Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.
    • 配列類似性
      Belongs to the peptidase C48 family.
    • 細胞内局在
      Nucleus. Cytoplasm. Shuttles between cytoplasm and nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • SENP 1 antibody
      • SENP1 antibody
      • SENP1_HUMAN antibody
      • Sentrin specific protease 1 antibody
      • Sentrin-specific protease 1 antibody
      • Sentrin/SUMO specific protease 1 antibody
      • Sentrin/SUMO specific protease antibody
      • Sentrin/SUMO specific protease SENP 1 antibody
      • Sentrin/SUMO specific protease SENP1 antibody
      • Sentrin/SUMO-specific protease SENP1 antibody
      • SUMO1/sentrin specific peptidase 1 antibody
      • SUMO1/sentrin specific protease 1 antibody
      • SuPr 2 antibody
      • SuPr2 antibody
      see all

    画像

    • Western blot analysis SENP 1 in whole HL60 cell lysate. SENP1 (Arrow) was detected using ab3656 (Lane B) but not pre-immune serum (lane A). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

    プロトコール

    参考文献

    This product has been referenced in:
    • Ni RS  et al. Detection of differentially expressed genes and association with clinicopathological features in laryngeal squamous cell carcinoma. Oncol Lett 4:1354-1360 (2012). WB ; Human . Read more (PubMed: 23226807) »
    • Dai XQ  et al. SUMOylation regulates insulin exocytosis downstream of secretory granule docking in rodents and humans. Diabetes 60:838-47 (2011). WB ; Human, Mouse . Read more (PubMed: 21266332) »

    See all 2 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human primary fibroblast)
    Gel Running Conditions
    Reduced Denaturing (3-8% gel)
    Loading amount
    5 µg
    Specification
    human primary fibroblast
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 18 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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