製品の概要

  • 製品名Anti-Semenogelin I antibody
    Semenogelin I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Semenogelin I
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Pig, Chimpanzee, African Green Monkey
  • 免疫原

    A region within synthetic peptide: ANDHDQSRKS QQYDLNALHK TTKSQRHLGG SQQLLHNKQE GRDHDKSKGH, corresponding to N terminal amino acids 73-122 of Human Semenogelin I

  • ポジティブ・コントロール
    • Jurkat cell lysate Human lung tissue Human kidney renal tubule

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.
    Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.
  • 組織特異性Seminal vesicle.
  • 配列類似性Belongs to the semenogelin family.
  • 翻訳後修飾Transglutaminase substrate.
    Rapidly cleaved after ejaculation by KLK3/PSA, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha-Inhibin-31 antibody
    • Alpha-Inhibin-92 antibody
    • Cancer/testis antigen 103 antibody
    • CT103 antibody
    • dJ172H20.2 (semenogelin I) antibody
    • dJ172H20.2 antibody
    • MGC14719 antibody
    • Semen coagulating protein antibody
    • Semenogelin antibody
    • Semenogelin I antibody
    • SEMG antibody
    • SEMG1 antibody
    • SEMG1_HUMAN antibody
    • Seminal basic protein antibody
    • seminal vesicle secretory protein 5 antibody
    • SgI antibody
    • Svp-1 antibody
    • Svp5 antibody
    • SVPIIA antibody
    • Svs2 antibody
    • SVS2P antibody
    • Svs2p2 antibody
    • Svs5 antibody
    see all

Anti-Semenogelin I antibody 画像

  • All lanes : Anti-Semenogelin I antibody (ab58692) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Semenogelin I at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Semenogelin I with ab58692 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-Semenogelin I antibody (ab58692) 使用論文

This product has been referenced in:
  • Teirilä L  et al. Proteomic changes of alveolar lining fluid in illnesses associated with exposure to inhaled non-infectious microbial particles. PLoS One 9:e102624 (2014). WB ; Human . Read more (PubMed: 25033447) »

See 1 Publication for this product

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Thank you for your reply.

It may indeed be easier to try this experiment with the rabbit monoclonal antibody ab139405. However, the immunogen information is proprietary information. In order to try to help you further could you explain what y...

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Thank you for contacting us and your interest in our products.

This is a very unusual question and I will try my best to help. The anti-Semenogelin I antibody ab58692 has been raised against a synthetic peptide taken from within the region yo...

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