Anti-Semaphorin 7a 抗体 [MEM-150] (FITC) (ab26012)

製品の概要

  • 製品名Anti-Semaphorin 7a antibody [MEM-150] (FITC)
    Semaphorin 7a 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-150] to Semaphorin 7a (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    T cell line HPB-ALL. (Human)

  • 特記事項

    The antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab26012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 20µl for 106 cells. Use 20 µl per 100 µl of whole blood.



ab91546-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.
  • 組織特異性Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney.
  • 配列類似性Belongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 PSI domain.
    Contains 1 Sema domain.
  • 細胞内局在Cell membrane. Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 108 antibody
    • CD108 antibody
    • CD108 antigen antibody
    • CDw108 antibody
    • H Sema K1 antibody
    • H Sema L antibody
    • H-SEMA-K1 antibody
    • H-Sema-L antibody
    • JMH antibody
    • JMH blood group antigen antibody
    • John Milton Hagen blood group H Sema K1 antibody
    • John Milton Hargen human blood group Ag antibody
    • John-Milton-Hargen human blood group Ag antibody
    • MGC126692 antibody
    • MGC126696 antibody
    • SEM7A_HUMAN antibody
    • Sema domain immunoglobulin domain (Ig) and GPI membrane anchor 7A antibody
    • Sema K1 antibody
    • Sema L antibody
    • SEMA7A antibody
    • SEMAK1 antibody
    • SEMAL antibody
    • Semaphorin 7A GPI membrane anchor antibody
    • Semaphorin K1 antibody
    • Semaphorin L antibody
    • Semaphorin-7A antibody
    • Semaphorin-K1 antibody
    • Semaphorin-L antibody
    • Semaphorin7a antibody
    see all

Anti-Semaphorin 7a antibody [MEM-150] (FITC) 画像

  • Overlay histogram showing Jurkat cells stained with ab26012 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26012, 1.2µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgGM FITC (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-Semaphorin 7a antibody [MEM-150] (FITC) (ab26012) 使用論文

ab26012 has not yet been referenced specifically in any publications.

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