Anti-Semaphorin 3F 抗体 (ab39956)

製品の概要

  • 製品名
    Anti-Semaphorin 3F antibody
    Semaphorin 3F 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Semaphorin 3F
  • アプリケーション
    適用あり: IHC-FoFr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Semaphorin 3F.

    (Peptide available as ab39955.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Brain (Rat); Brain (Mouse); Brain (Human)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab39956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr
WB
  • 追加情報
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 88,120 kDa (predicted molecular weight: 88 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      May play a role in cell motility and cell adhesion.
    • 組織特異性
      Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.
    • 配列類似性
      Belongs to the semaphorin family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Sema domain.
    • 発生段階
      Detected as early as embryonic day 10.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • SEM3F_HUMAN antibody
      • sema domain immunoglobulin domain Ig short basic domain secreted 3F antibody
      • sema domain immunoglobulin domain Ig short basic domain secreted semaphorin 3F antibody
      • Sema III/F antibody
      • Sema IV antibody
      • SEMA-IV antibody
      • SEMA3F antibody
      • SEMA4 antibody
      • SemaIII/F antibody
      • SemaIV antibody
      • SEMAK antibody
      • Semaphorin IV antibody
      • Semaphorin-3F antibody
      • Semaphorin3F antibody
      • SemaphorinIV antibody
      see all

    Anti-Semaphorin 3F antibody 画像

    • All lanes : Anti-Semaphorin 3F antibody (ab39956) at 1 µg/ml

      Lane 1 : Human brain tissue lysate - total protein (ab29466)
      Lane 2 : Brain (Rat) Tissue Lysate
      Lane 3 : Brain (Mouse) Tissue Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 88 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 30 kDa,37 kDa,45 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 4 minutes

    Anti-Semaphorin 3F antibody (ab39956) 使用論文

    ab39956 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
    Sample
    Mouse Cell (Brain sections)
    Specification
    Brain sections
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jul 24 2014

    Application
    Western blot
    Loading amount
    200000 cells
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Sample
    Mouse Cell lysate - whole cell (Brain tissue)
    Specification
    Brain tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 13 2014

    Thank you for your reply. My colleague you were in correspondence with is on holiday at the moment. I checked again for you and I have to confirm that ab39955 (Semaphorin 3F peptide) is only a short peptide of 18 amino acids (used a immunogen to genera...

    Read More

    Thank you for contacting us. As you mention, we do have a number of related products available. The SEMA3F products we have in our catalog (such as ab39956) is a rabbit-polyclonal antibody against Semaphorin 3F. It is guaranteed to work with mo...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (brain sections)
    Specification
    brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Username

    Dr. Sophie Pezet

    Verified customer

    投稿 Oct 01 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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