The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.03 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 89 kDa). Preliminary experiments gave an approx 35kDa band in Human Prostate lysates. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
Belongs to the semaphorin family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Sema domain.
Mazzotta C et al. Plexin-D1/Semaphorin 3E pathway may contribute to dysregulation of vascular tone control and defective angiogenesis in systemic sclerosis. Arthritis Res Ther17:221 (2015).
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