製品の概要

  • 製品名
    Anti-Semaphorin 3B antibody
    Semaphorin 3B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Semaphorin 3B
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Semaphorin 3B.

    (Peptide available as ab101720.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human brain tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 83 kDa).

ターゲット情報

  • 機能
    Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
  • 組織特異性
    Expressed abundantly but differentially in a variety of neural and nonneural tissues.
  • 配列類似性
    Belongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 PSI domain.
    Contains 1 Sema domain.
  • 細胞内局在
    Secreted. Endoplasmic reticulum. Accumulates in the endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • SEM3B_HUMAN antibody
    • Sema A(V) antibody
    • Sema V antibody
    • Sema3b antibody
    • Semaphorin V antibody
    • Semaphorin-3B antibody
    • Semaphorin-V antibody
    see all

画像

  • Anti-Semaphorin 3B antibody (ab88489) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

プロトコール

参考文献

ab88489 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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