製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SEMA4B
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 182-231 (KGRCPFDPNF KSTALVVDGE LYTGTVSSFQ GNDPAISRSQ SLRPTKTESS) of human SEMA4B (NP_064595).

  • ポジティブ・コントロール
    • Fetal membrane lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified by peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab81130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    SEMA4B belongs to the semaphorin family and contains 1 Ig-like C2-type (immunoglobulin-like) domain, 1 PSI domain and 1 Sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
  • 細胞内局在
    Membrane
  • 参照データベース
  • 別名
    • KIAA1745 antibody
    • MGC131831 antibody
    • Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4B antibody
    • Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain 4B antibody
    • SEMA4B antibody
    • SEMAC antibody
    • Semaphorin 4B antibody
    • SemC antibody
    see all

Anti-SEMA4B antibody 画像

  • Anti-SEMA4B antibody (ab81130) at 1 µg/ml + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Anti-SEMA4B antibody (ab81130) at 1.6 µg/ml + MIA-PACA2 cells

    Predicted band size : 93 kDa

    WB Suggested Anti-WBP11 antibody (ab85563) Titration: 2 µg/ml Positive Control: Human NT-2 cells and Mouse WT brain.

    Secondary antibody - anti-rabbit HRP (ab6721)

プロトコール

Anti-SEMA4B antibody (ab81130) 使用論文

ab81130 has not yet been referenced specifically in any publications.

Product Wall

Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
No
Sample
Mouse Cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Gating Strategy
live cells in fsc vs ssc
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.2% BSA, 0.1% NaAz in PBS
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投稿 Apr 30 2014

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Jan 07 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (HEK293 cells transfected with mouse Sema4B express)
Specification
HEK293 cells transfected with mouse Sema4B express
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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投稿 Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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