Anti-SEMA4A 抗体 (ab70178)
Key features and details
- Rabbit polyclonal to SEMA4A
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-SEMA4A antibody
SEMA4A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SEMA4A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, African green monkey
交差が予測される動物種: Mouse, Rat -
免疫原
A synthesized peptide derived from the internal region of human SEMA4A.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70178の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 84 kDa.
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IHC-P |
1/50 - 1/100.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 84 kDa. |
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. -
関連疾患
Defects in SEMA4A are the cause of retinitis pigmentosa type 35 (RP35) [MIM:610282]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Defects in SEMA4A are the cause of cone-rod dystrophy type 10 (CORD10) [MIM:610283]. CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa. -
配列類似性
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 64218 Human
- Entrez Gene: 20351 Mouse
- Entrez Gene: 310630 Rat
- Omim: 607292 Human
- SwissProt: Q9H3S1 Human
- SwissProt: Q62178 Mouse
- Unigene: 408846 Human
- Unigene: 439752 Mouse
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別名
- CORD10 antibody
- RP11 54H19 2 antibody
- RP35 antibody
see all
画像
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All lanes : Anti-SEMA4A antibody (ab70178) at 1/500 dilution
Lane 1 : Extracts from COS-7 cells
Lane 2 : Extracts from Jurkat cells
Lane 3 : Extracts from COS-7 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Additional bands at: 117 kDa, 26 kDa. We are unsure as to the identity of these extra bands.
One other band detected greater then 117 kDa. -
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab70178 at 1/50-1/100 dilution.
Left image un-treated.Right image treated with immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab70178 は 3 報の論文で使用されています。
- Sukumaran SK et al. Whole transcriptome profiling of taste bud cells. Sci Rep 7:7595 (2017). PubMed: 28790351
- Wang L et al. Expression of Semaphorin 4A and its potential role in rheumatoid arthritis. Arthritis Res Ther 17:227 (2015). ELISA ; Human . PubMed: 26303122
- Vadasz Z et al. The involvement of immune semaphorins and neuropilin-1 in lupus nephritis. Lupus 20:1466-73 (2011). IHC-P ; Human . PubMed: 21951945