Rabbit polyclonal to Selenophosphate synthetase 2
Mouse, Rat, Cow, Dog, Pig, Monkey
Recombinant fragment corresponding to a region within amino acids 186-422 of Human Selenophosphate synthetase 2 (AAH16643).
HeLa S3 whole cell lysate; HeLa cells; 293T cell line
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
1/100 - 1/200.
This protein encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co translationally incorporated into selenoproteins at in frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop.
Selenide, water dikinase 2 antibody
Selenium donor protein 2 antibody
selenophosphate synthetase 2 antibody
Western blot - Selenophosphate synthetase 2 antibody (ab96541)
Anti-Selenophosphate synthetase 2 antibody (ab96541) at 1/1000 dilution + Hela S3 whole cell lysate at 30 µg
Predicted band size : 47 kDa
Immunocytochemistry/ Immunofluorescence - Selenophosphate synthetase 2 antibody (ab96541)
ab96541, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by Immunofluorescence (Panel 1) and merged with DNA probe (Panel 2).
has not yet been referenced specifically in any publications.
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