製品の概要

  • 製品名Anti-Securin antibody [DCS-280]
    Securin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [DCS-280] to Securin
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Human recombinant full length Pds 1 protein.

  • ポジティブ・コントロール
    • HeLa cells. SVEC4-10 cells. Tonsil tissue sections.
  • 特記事項LocusID: 9232 OMIM: 604147 SwissProt accession no.: O95997 Alternative names: Pituitary Tumor Transforming Gene 1; PTTG1; PTTG; Esp1-associated protein 1; EAP 1; Tumor-Transforming 1; TUTR 1; Securin; Pds 1; Pds 1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50 - 1/200.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration. See Abreview.

ターゲット情報

  • 機能Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
  • 組織特異性Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.
  • 配列類似性Belongs to the securin family.
  • 発生段階Low level during G1 and S phases. Peaks at M phase. During anaphase, it is degraded.
  • ドメインThe N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
    The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thiolester with the acceptor lysine residue.
  • 翻訳後修飾Phosphorylated at Ser-165 by CDK1 during mitosis.
    Phosphorylated in vitro by ds-DNA kinase.
    Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AW555095 antibody
    • C87862 antibody
    • Cut2 antibody
    • EAP 1 antibody
    • EAP1 antibody
    • ESP1 associated protein 1 antibody
    • Esp1-associated protein antibody
    • hPTTG antibody
    • MGC126883 antibody
    • MGC138276 antibody
    • Pds1 antibody
    • Pituitary tumor transforming 1 antibody
    • Pituitary tumor transforming protein 1 antibody
    • Pituitary tumor-transforming 1, isoform CRA_a antibody
    • Pituitary tumor-transforming 1, isoform CRA_b antibody
    • Pituitary tumor-transforming gene 1 antibody
    • Pituitary tumor-transforming gene 1 protein antibody
    • PTTG 1 antibody
    • PTTG antibody
    • PTTG1 antibody
    • PTTG1 protein antibody
    • PTTG1_HUMAN antibody
    • Pttg3 antibody
    • Securin antibody
    • Tumor transforming 1 antibody
    • Tumor transforming protein 1 antibody
    • Tumor-transforming protein 1 antibody
    • TUTR 1 antibody
    • TUTR1 antibody
    see all

Anti-Securin antibody [DCS-280] 画像

  • ab3305 (1/200) staining Securin in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde (4%, 10 minutes), permeabilised (0.5% Triton X100 in PBS) and counterstained with DAPI in order to highlight the nucleus (red). Please refer to Abreview for further experimental details.

    See Abreview

  • ab3305 (1µg/ml) staining Securin in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and nuclear staining of cells of the germinal centre.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Anti-Securin antibody [DCS-280] (ab3305) at 2 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes
  • All lanes : Anti-Securin antibody [DCS-280] (ab3305) at 1/1000 dilution

    Lane 1 : AtT20 (mouse pituitary) cell lysate
    Lane 2 : AtT20 (mouse pituitary) cell lysate
    Lane 3 : AtT20 (mouse pituitary) cell lysate
    Lane 4 : AtT20 (mouse pituitary) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated Sheep anti-Mouse polyclonal at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 27 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    This image is courtesy of an Anonymous Abreview.

    Cells were transfected with different genes.

    See Abreview

  • Anti-Securin antibody [DCS-280] (ab3305) at 1/1000 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    HRP-conjugated goat anti-mouse polyclonal IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 5% BSA for 2 hours at 25°C

    See Abreview

Anti-Securin antibody [DCS-280] (ab3305) 使用論文

This product has been referenced in:
  • Heikkinen I  et al. Does securin expression have significance in prognostication of oral tongue cancer? A pilot study. Eur Arch Otorhinolaryngol 273:3905-3911 (2016). Read more (PubMed: 27091335) »
  • Kurkalang S  et al. Precocious anaphase and expression of Securin and p53 genes as candidate biomarkers for the early detection in areca nut-induced carcinogenesis. Mutagenesis 30:381-9 (2015). Read more (PubMed: 25480871) »

See all 39 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA/Tris
Sample Dog Tissue sections (Testes)
Specification Testes
Permeabilization No
Fixative 10% NBF
Username

Mrs. Susan Shapiro

Verified customer

投稿 Jan 26 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample Human Cell (HT1080)
Specification HT1080
Permeabilization Yes - 0.5% Triton X100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 14 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Denaturing (18%)
Sample Human Cell lysate - whole cell (HT1080)
Specification HT1080
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jan 14 2015

Thank you for your inquiry.

I can recommend to use ab79546 (Anti-Securin antibody [EPR3240]) for your flow cytometry experiments.

This antibody is tested and guaranteed for flow cytometry in human samples.

http://www.abcam...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 50 µg
Specification Hela
Gel Running Conditions Reduced Denaturing (12)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 30 2012

Thank you for visiting the Abcam booth at ASCB. I am sorry to hear that you have experienced difficulties with our Securin antibody, ab3305.  I was hoping you would provide your protocol information for our records so that we may investigate this p...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (pituitary cell line)
Loading amount 20 µg
Specification pituitary cell line
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Sep 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Rat pituitary cells)
Loading amount 20 µg
Specification Rat pituitary cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Sep 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (MDA-MB-231 breast cancer cell line)
Total protein in input 40 µg
Specification MDA-MB-231 breast cancer cell line
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

投稿 Oct 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Mar 07 2008

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"