製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Securin
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性

    交差が予測される動物種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Securin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HEK293 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab26273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).
ICC/IF 1/100.

ターゲット情報

  • 機能
    Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
  • 組織特異性
    Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.
  • 配列類似性
    Belongs to the securin family.
  • 発生段階
    Low level during G1 and S phases. Peaks at M phase. During anaphase, it is degraded.
  • ドメイン
    The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
    The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thiolester with the acceptor lysine residue.
  • 翻訳後修飾
    Phosphorylated at Ser-165 by CDK1 during mitosis.
    Phosphorylated in vitro by ds-DNA kinase.
    Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AW555095 antibody
    • C87862 antibody
    • Cut2 antibody
    • EAP 1 antibody
    • EAP1 antibody
    • ESP1 associated protein 1 antibody
    • Esp1-associated protein antibody
    • hPTTG antibody
    • MGC126883 antibody
    • MGC138276 antibody
    • Pds1 antibody
    • Pituitary tumor transforming 1 antibody
    • Pituitary tumor transforming protein 1 antibody
    • Pituitary tumor-transforming 1, isoform CRA_a antibody
    • Pituitary tumor-transforming 1, isoform CRA_b antibody
    • Pituitary tumor-transforming gene 1 antibody
    • Pituitary tumor-transforming gene 1 protein antibody
    • PTTG 1 antibody
    • PTTG antibody
    • PTTG1 antibody
    • PTTG1 protein antibody
    • PTTG1_HUMAN antibody
    • Pttg3 antibody
    • Securin antibody
    • Tumor transforming 1 antibody
    • Tumor transforming protein 1 antibody
    • Tumor-transforming protein 1 antibody
    • TUTR 1 antibody
    • TUTR1 antibody
    see all

画像

  • HeLa cells stained with ab26273 (1/100 dilution). A clear nuclear signal in interphase HeLa cells can be seen.


  • Predicted band size : 22 kDa

    This image is courtesy of an abreview submitted by Andrew Horwitz

    See Abreview

  • Anti-Securin antibody (ab26273) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 minutesab26273 detects a clean 26 kDa band in HEK293 lysate. We would recommend loading 20-40ug of protein when using this antibody. ab26273 has also been tested in mouse lysates and in our hands detects a 70 kDa band which we cannot explain.

参考文献

ab26273 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
50 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (12% Bis-TRIS in MOPS buffer)
Blocking step
Odyssey Blocking Agent (Lycor system) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Feb 05 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (sPhi992 strain)
Loading amount
50 µg
Specification
sPhi992 strain
Gel Running Conditions
Reduced Denaturing (12% Bis-TRIS in MOPS buffer)
Blocking step
Odyssey Blocking Agent (Lycor system) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Feb 05 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MPRO (mouse promyelocytic) cells)
Loading amount
50 µg
Specification
MPRO (mouse promyelocytic) cells
Gel Running Conditions
Reduced Denaturing (12% Bis-TRIS in MOPS buffer)
Blocking step
Odyssey Blocking Agent (Lycor system) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 05 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 29 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa MCF7 Hs578T)
Loading amount
20 µg
Specification
HeLa MCF7 Hs578T
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Mar 12 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Oct 03 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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