Anti-Secretory phospholipase A2 Type V 抗体 (ab23709)


  • 製品名Anti-Secretory phospholipase A2 Type V antibody
    Secretory phospholipase A2 Type V 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Secretory phospholipase A2 Type V
  • 特異性(+) Human, murine, and rat sPLA2 (Type V); (-) bee venom and human synovial sPLA2 (Type II), iPLA2 (A10 cell lysate), and cPLA2 (HeLa cell lysate); sPLA2 (Type X) not tested, but is expected to be negative (peptide antigen is 12.5% homologous to murine and rat sPLA2 (Type X) and 25% homologous to human (Type II) and (Type X) sPLA2).
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 79-94 of Mouse Secretory phospholipase A2 Type V.

  • ポジティブ・コントロール
    • Expressed in heart, lung, placenta and spleen. IHC-P: FFPE human heart tissue sections.



Our Abpromise guarantee covers the use of ab23709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
  • 追加情報WB: 1/500. Predicted molecular weight: 15 kDa (Human).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle.
    • 組織特異性Heart, placenta and less abundantly, in lung.
    • 配列類似性Belongs to the phospholipase A2 family.
    • 翻訳後修飾This enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Ca2+ dependent phospholipase A2 antibody
      • Calcium dependent phospholipase A2 antibody
      • Calcium-dependent phospholipase A2 antibody
      • DKFZp686C2294 antibody
      • FRFB antibody
      • Group V phospholipase A2 antibody
      • GV PLA2 antibody
      • gVPLA2 antibody
      • hVPLA(2) antibody
      • MGC46205 antibody
      • OTTHUMP00000044655 antibody
      • PA2G5_HUMAN antibody
      • Phosphatidylcholine 2 acylhydrolase antibody
      • Phosphatidylcholine 2-acylhydrolase 5 antibody
      • Phospholipase A2 group V antibody
      • PLA2 10 antibody
      • PLA2 G5 antibody
      • PLA2-10 antibody
      • Pla2g5 antibody
      • sPLA2 Type V antibody
      see all

    Anti-Secretory phospholipase A2 Type V antibody 画像

    • IHC image of Secretory phospholipase A2 Type V staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23709, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-Secretory phospholipase A2 Type V antibody (ab23709) at 1/500 dilution

      Lane 1 : Recombinant human sPLA2 (type V) (100 ng)
      Lane 2 : Recombinant human sPLA2 (type V) (50 ng)
      Lane 3 : Mouse heart homogenate (50ug)

      Predicted band size : 15 kDa
      Observed band size : 15 kDa

    Anti-Secretory phospholipase A2 Type V antibody (ab23709) 使用論文

    ab23709 has not yet been referenced specifically in any publications.

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