製品の概要

  • 製品名Anti-Secretogranin V antibody
    Secretogranin V 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Secretogranin V
  • アプリケーション適用あり: IP, ICC/IF, WB, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Rat, Human, Pig
    交差が予測される動物種: Mouse, Chicken, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:

    EQLGIARPRV EYPAHQA

    conjugated to BSA by a CDI linker, corresponding to N terminal amino acids 23-39 of Pig Secretogranin V (45-61 of pro-Pig Secretogranin V).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab22699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Detects a band of approximately 25 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 20434189

ターゲット情報

  • 関連性Known variously as APPG, 7B2 and neuroendocrine protein I, Secretogranin V was originally isolated from porcine pituitary in 1983. It soon became evident that Secretogranin V-like immunoreactants were present in many cell types from a variety of species, and it is now recognised to be widely distributed in neural and endocrine tissues. In the early 1990s full length (27kDa) Secretogranin V was shown to be a potent inhibitor of prohormone convertase PC2 activity, a subtilisin-like enzyme responsible for the proteolytic cleavage of many neuroendocrine precursor molecules, including proenkephalin, POMC and gastrin-17. mRNA distribution analysis revealed that in adult rat brain, expression of Secretogranin V mRNA was pan-neuronal, whereas PC2 mRNA was exclusively neuronal, but more restricted. PC2+/Secretogranin V- cells were not encountered in the adult, but were common in developing rat brain. These findings suggest that Secretogranin V may have intracellular functions other than PC2 maturation in certain cells, although some recent work shows a discordancy between mRNA expression and enzymic activity. The mechanism for PC2 maturation appears to involve pro-Secretogranin V binding to inactive pro-PC2 via its polyproline motif (116 PPNPCP 121) in the endoplasmic reticulum and chaperoning pro-PC2 to a later secretory pathway compartment where maturation of PC2 proceeds once pro-Secretogranin V is proteolytically processed by a furin-like convertase into a 21kDa fragment and a C-terminal peptide. It is the C-terminal peptide that potently inhibits maturation of pro-PC2 until the complex is subjected to a decreasing pH gradient along the secretory pathway. It seems likely that mature PC2 then cleaves the CT peptide at its internal lys-lys site. Secretogranin V knockout mice develop a severe Cushings-like phenotype and exhibit multiple metabolic and hormonal abnormalities, indicating that Secretogranin V is required for activation of PC2 in vivo. Conversely, PC2-null mice appear to be viable.
  • 細胞内局在Neuroendocrine and endocrine secretory granules.
  • 参照データベース
  • 別名
    • 7B2 antibody
    • 7B2 protein antibody
    • APPG antibody
    • Neuroendocrine protein 7B2 antibody
    • P7B2 antibody
    • Pituitary polypeptide antibody
    • Prohormone convertase chaperone antibody
    • SCG 5 antibody
    • SCG5 antibody
    • Secretogranin 5 antibody
    • Secretogranin V 7B2 protein antibody
    • Secretogranin5 antibody
    • SecretograninV antibody
    • Secretory granule endocrine protein I antibody
    • Secretory granule neuroendocrine protein 1 7B2 protein antibody
    • Secretory granule neuroendocrine protein 1 antibody
    • Sg V antibody
    • SGNE 1 antibody
    • SGNE1 antibody
    • SgV antibody
    see all

Anti-Secretogranin V antibody 画像

  • ab75197 (1µg/ml) staining secretogranin V in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of Islets of Langerhans.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab22699 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22699, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Secretogranin V antibody (ab22699) 使用論文

This product has been referenced in:
  • Sato A  et al. Distribution of 7B2 (secretogranin V)-like immunoreactivity in the Japanese red-bellied newt (Cynops pyrrhogaster) pituitary. Tissue Cell 42:176-80 (2010). IHC-Fr ; Newt . Read more (PubMed: 20434189) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Kidney Tissue)
Permeabilization Yes - PBS+0.1% Triton
Specification Kidney Tissue
Blocking step Super Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Vijay Saxena

Verified customer

投稿 May 11 2016

Thank you for contacting Abcam Because the ab22699 antibody is only partially purified, the antibody concentration is not determined, and therefore is unknown. I am sorry that we are not able to provide more information. Please let me know if I can be ...

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Thank you for contacting Abcam. Due to the holidays, it will be next week before I get a response from the lab about the concentration of ab22699. I will get back to you as soon as I can with that information. Have a Happy New Year.

Thank you for contacting Abcam. It is possible that ab22699 could be used as a granule marker however it may also react with other structures. I have included a reference below that may prove useful: Sato Aet al. Distribution of 7B2 (secretogranin V)-l...

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Application Western blot
Sample Rat Cell lysate - whole cell (INS-1 pancreatic cells)
Loading amount 20 µg
Specification INS-1 pancreatic cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Username

Dr. Sarah Ronnebaum

Verified customer

投稿 Aug 07 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"