Anti-SEC61B 抗体 (ab15576)
Key features and details
- Rabbit polyclonal to SEC61B
- Suitable for: ICC/IF
- Reacts with: Mouse, Rat, Dog, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-SEC61B antibody
SEC61B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SEC61B -
由来種
Rabbit -
特異性
Ab15576 detects human, dog, pig, and rat sec61 beta from microsomes and shows no cross-reactivity with other sec61 isoforms. -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Dog, Human, Pig -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- ICC/IF: MDCK cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15576の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Necessary for protein translocation in the endoplasmic reticulum. -
配列類似性
Belongs to the SEC61-beta family. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 404018 Dog
- Entrez Gene: 10952 Human
- Entrez Gene: 66212 Mouse
- Entrez Gene: 298068 Rat
- Omim: 609214 Human
- SwissProt: P60467 Dog
- SwissProt: P60468 Human
- SwissProt: Q9CQS8 Mouse
see all -
別名
- Protein translocation complex beta antibody
- Protein transport protein SEC61 beta subunit antibody
- Protein transport protein Sec61 subunit beta antibody
see all
画像
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Immunofluorescence analysis of SEC61B was performed using 70% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SEC61B Rabbit Polyclonal Antibody (ab15576) at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab15576 は 4 報の論文で使用されています。
- Xu F et al. COPII mitigates ER stress by promoting formation of ER whorls. Cell Res 31:141-156 (2021). PubMed: 32989223
- Bogdanov V et al. Distributed synthesis of sarcolemmal and sarcoplasmic reticulum membrane proteins in cardiac myocytes. Basic Res Cardiol 116:63 (2021). PubMed: 34713358
- Liu X et al. The BEACH-containing protein WDR81 coordinates p62 and LC3C to promote aggrephagy. J Cell Biol 216:1301-1320 (2017). PubMed: 28404643
- Zhang R et al. A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. Nature 535:164-8 (2016). PubMed: 27383988