Anti-Sec31A 抗体 (ab86600)
Key features and details
- Rabbit polyclonal to Sec31A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Sec31A antibody
Sec31A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Sec31A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Pig, Chimpanzee, Ferret, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human Sec31A aa 750-850.
Database link: O94979 -
ポジティブ・コントロール
- HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86600の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性
The protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII, in the subcellular structures corresponding to the vesicle transport function. -
細胞内局在
Cytoplasm. Cytoplasmic vesicle; COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Note: Associates with membranes in a GTP-dependent manner -
参照データベース
- Entrez Gene: 22872 Human
- Omim: 610257 Human
- SwissProt: O94979 Human
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別名
- 1810024J13Rik antibody
- ABP125 antibody
- ABP130 antibody
see all
画像
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All lanes : Anti-Sec31A antibody (ab86600) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 133 kDa
Observed band size: 133 kDa
Exposure time: 3 seconds -
Detection of Sec31A by Western Blot of Immunprecipitate.
ab86600 at 0.1µg/ml staining Sec31A in HeLa whole cell lysate immunoprecipitated using ab86600 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab86600 は 1 報の論文で使用されています。
- Morgan BM et al. Nonsynonymous SNPs in LPA homologous to plasminogen deficiency mutants represent novel null apo(a) alleles. J Lipid Res 61:432-444 (2020). PubMed: 31806727