製品の概要

  • 製品名Anti-SEC16A antibody
    SEC16A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SEC16A
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog, Ferret, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residues 600 and 650 of human SEC16A. (SwissProt O15027).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa and 293T cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately >250 kDa (predicted molecular weight: 234 kDa).
IP Use at 1-4 µg/mg of lysate.

ターゲット情報

  • 関連性SEC16A is required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus. It defines endoplasmic reticulum exit sites (ERES). SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. It is required for normal transitional endoplasmic reticulum (tER) organization.
  • 細胞内局在Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Note=SAR1A activity is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation.
  • 参照データベース
  • 別名
    • FLJ26737 antibody
    • KIAA0310 antibody
    • OTTHUMP00000022589 antibody
    • OTTHUMP00000022592 antibody
    • OTTHUMP00000022593 antibody
    • p250 antibody
    • Protein SEC16 homolog A antibody
    • Protein transport protein Sec16A antibody
    • RP11 413M3.10 antibody
    • SEC 16 antibody
    • SEC 16A antibody
    • SEC16 antibody
    • SEC16 homolog A (S. cerevisiae) antibody
    • SEC16 homolog A antibody
    • SEC16L antibody
    see all

Anti-SEC16A antibody 画像

  • All lanes : Anti-SEC16A antibody (ab70722) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 234 kDa
    Observed band size : >250 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of Human SEC16A by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70722 at 3 µg/mg lysate. Subsequent Western blot detection of SEC16A was performed using ab70722 at 0.04 µg/ml.

Anti-SEC16A antibody (ab70722) 使用論文

ab70722 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Kidney)
Permeabilization Yes - 0.1% triton x-100
Specification Kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
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投稿 Jun 17 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12)
Loading amount 30 µg
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Apr 08 2016

Application Western blot
Sample Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Apr 08 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
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投稿 Feb 23 2016

Application Western blot
Sample Human Cell lysate - whole cell (lymphocytes)
Gel Running Conditions Reduced Denaturing (4-12% Bis-tris)
Loading amount 15 µg
Specification lymphocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Oct 16 2015

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 22000 cells
Gel Running Conditions Reduced Denaturing (NuPAGE® Novex® 4-12% Bis-Tris Gel,1.0 mm)
Sample Human Cell lysate - whole cell (HeLa cells)
Specification HeLa cells
Treatment different siRNAs for 72h
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 May 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"