製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to SDPR
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-425 of Human SDPR (NP_004648)

  • ポジティブ・コントロール
    • transfected 293T cell line

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.

ターゲット情報

  • 機能
    May play a role in targeting PRKCA to caveolae.
  • 組織特異性
    Highly expressed in heart and lung, and expressed at lower levels in brain, kidney, liver, pancreas, placenta, and skeletal muscle.
  • 配列類似性
    Belongs to the PTRF/SDPR family.
  • 翻訳後修飾
    Phosphorylated on Ser residues.
  • 細胞内局在
    Cytoplasm > cytosol. Membrane > caveola. Colocalizes with CAV1 to caveolae.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cavin 2 antibody
    • Cavin-2 antibody
    • Phosphatidylserine binding protein antibody
    • Phosphatidylserine-binding protein antibody
    • PS p68 antibody
    • PS-p68 antibody
    • Sdpr antibody
    • SDPR_HUMAN antibody
    • SDR antibody
    • Serum deprivation response antibody
    • Serum deprivation response protein antibody
    • Serum deprivation-response protein antibody
    see all

Anti-SDPR antibody 画像

  • All lanes : Anti-SDPR antibody (ab76867) at 1/500 dilution

    Lane 1 : SDPR transfected 293T cell line
    Lane 2 : non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 47 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)

プロトコール

Anti-SDPR antibody (ab76867) 使用論文

ab76867 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (Skeletal muscle cells)
Specification
Skeletal muscle cells
Treatment
N/A
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 21 2014

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Stem Cells (ES-E14TG2aATCC))
Loading amount
30 µg
Specification
Mouse Embryonic Stem Cells (ES-E14TG2aATCC)
Treatment
100 µg/ml fibronectin for 24 hrs
Gel Running Conditions
Non-reduced Denaturing (gel 10 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Aug 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録