製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ICC Use a concentration of 5 µg/ml. Requires heat-induced antigen retrieval where aldehydes are used as fixatives.
IHC-P Use at an assay dependent concentration. PubMed: 20236688
WB Use a concentration of 5 µg/ml. Detects a band of approximately 28 kDa.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
  • パスウェイCarbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
  • 関連疾患Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
    Defects in SDHB are the cause of hereditary paragangliomas type 4 (PGL4) [MIM:115310]; also known as familial non-chromaffin paragangliomas type 4. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. PGL4 is characterized by the development of mostly benign, highly vascular, slow growing tumors in the head and neck. In the head and neck region, the carotid body is the largest of all paraganglia and is also the most common site of the tumors.
    Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance.
    Defects in SDHB are a cause of Cowden-like syndrome (CWDLS) [MIM:612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus.
  • 配列類似性Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
    Contains 1 2Fe-2S ferredoxin-type domain.
    Contains 1 4Fe-4S ferredoxin-type domain.
  • 細胞内局在Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CWS2 antibody
    • DHSB_HUMAN antibody
    • FLJ92337 antibody
    • Ip antibody
    • Iron sulfur subunit antibody
    • Iron sulfur subunit of complex II antibody
    • Iron-sulfur subunit of complex II antibody
    • mitochondrial antibody
    • PGL 4 antibody
    • PGL4 antibody
    • SDH 1 antibody
    • SDH antibody
    • SDH1 antibody
    • SDH2 antibody
    • SDH2, homolog of antibody
    • SdhB antibody
    • SDHIP antibody
    • Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial antibody
    • Succinate dehydrogenase [ubiquinone] iron-sulfur subunit antibody
    • succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial antibody
    • Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody
    • Succinate Dehydrogenase 2, S. cerevisiae, homolog of antibody
    • Succinate dehydrogenase complex subunit B iron sulfur antibody
    • Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody
    • succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody
    • Succinate dehydrogenase iron sulfur protein antibody
    see all

Anti-SDHB antibody [21A11AE7] 画像

  • All lanes : Anti-SDHB antibody [21A11AE7] (ab14714)

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine Heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
    Lane 5 : Isolated mitochondria from HepG2 cells at 20 µg

    Secondary
    Goat anti-Mouse secondary

    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
    Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
  • ab14714 at 5ug/ml staining human adrenal tumor tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 1 hour. A biotinylated horse anti-mouse IgG antibody was used as the secondary.

    See Abreview

  • ab14714 staining SDHB in normal ageing human colon tissue by Immunohistochemistry (Frozen sections).
  • ICC/IF image of ab14714 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14714, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Mitochondrial localization of complex II visualized by immunocytochemistry using anti-complex II subunit 30 kDa Ip mAb 21A11 (ab14714). Cells were fixed, permeabilized and then labeled with ab14714 followed by an Alexa Fluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody.
  • Overlay histogram showing HEK293 cells stained with ab14714 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14714, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-SDHB antibody [21A11AE7] (ab14714) 使用論文

This product has been referenced in:
  • Pérez-Pérez R  et al. COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation. Cell Rep 16:2387-98 (2016). WB . Read more (PubMed: 27545886) »
  • Bezawork-Geleta A  et al. The Assembly Factor SDHAF2 Is Dispensable for Flavination of the Catalytic Subunit of Mitochondrial Complex II in Breast Cancer Cells. J Biol Chem 291:21414-21420 (2016). Read more (PubMed: 27587393) »

See all 82 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (flight muscles)
Gel Running Conditions Reduced Denaturing
Loading amount 40 µg
Specification flight muscles
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
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投稿 Jul 06 2016

Application Western blot
Sample Chinese Hamster Cell lysate - whole cell (CHO cell)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification CHO cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Aug 13 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% GEL)
Sample Human Cell lysate - whole cell (H460)
Specification H460
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
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投稿 May 19 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (15% GEL)
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts)
Specification Mouse Embryonic Fibroblasts
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
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投稿 May 19 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Sample Human Cell (H460)
Specification H460
Permeabilization Yes - 0.5% Triton-X
Fixative Paraformaldehyde
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投稿 May 19 2014

Application Immunocytochemistry
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Sample Mouse Cultured Cells (Mouse Embryonic Fibroblasts)
Specification Mouse Embryonic Fibroblasts
Permeabilization Yes - 0.5% Triton-X
Fixative Paraformaldehyde
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投稿 May 19 2014

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (hek293)
Specification hek293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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投稿 May 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (h9c2 cell)
Loading amount 30 µg
Specification h9c2 cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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投稿 Mar 12 2013

Now that MitoSciences is part of Abcam, we don’t offer test vials any more. However, as this antibody is a monoclonal, there shouldn’t be significant batch-batch variation.

Please note as well that we currently only have 1 lot in ...

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