製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SCYL2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-929 of Human SCYL2 (NP_060458.3).

  • ポジティブ・コントロール
    • Jurkat cell line lysate. SCYL2 transfected 293T cell line lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab103819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 104 kDa.

ターゲット情報

  • 機能
    Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system.
  • 配列類似性
    Belongs to the protein kinase superfamily.
    Contains 1 HEAT repeat.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    According to PubMed:15809293, autophosphorylated in presence of poly-L-lysine.
  • 細胞内局在
    Cytoplasm > perinuclear region. Cytoplasmic vesicle > clathrin-coated vesicle. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. According to PubMed:15809293, plasma membrane-associated in clathrin-coated vesicles. According to PubMed:16914521, colocalizes to the trans-Golgi network (TGN) and to endosomal membranes with clathrin, transferrin and plasma membrane adapter AP1 and AP3 complexes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AU015688 antibody
    • AW536445 antibody
    • BC030932 antibody
    • Coated vesicle-associated kinase of 104 kDa antibody
    • CVAK104 antibody
    • D10Ertd802e antibody
    • FLJ10074 antibody
    • KIAA1360 antibody
    • RGD1305665 antibody
    • SCY1 like 2 (S. cerevisiae) antibody
    • SCY1 like protein 2 antibody
    • SCY1-like protein 2 antibody
    • SCYL2 antibody
    • SCYL2_HUMAN antibody
    see all

画像

  • Anti-SCYL2 antibody (ab103819) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 104 kDa
  • All lanes : Anti-SCYL2 antibody (ab103819) at 1 µg/ml

    Lane 1 : SCYL2 transfected 293T cell line lysate
    Lane 2 : Non transfected 293T cell line lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 104 kDa

プロトコール

参考文献

This product has been referenced in:
  • Wojcechowskyj JA  et al. Quantitative phosphoproteomics reveals extensive cellular reprogramming during HIV-1 entry. Cell Host Microbe 13:613-23 (2013). WB ; Human . Read more (PubMed: 23684312) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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