Regulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro. Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region.
Belongs to the protein kinase superfamily. Contains 3 HEAT repeats. Contains 1 protein kinase domain.
The protein kinase domain is predicted to be catalytically inactive.
Nucleus; Cytoplasm > cytoskeleton > centrosome. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus > cis-Golgi network. Localized to the Endoplasmic reticulum-Golgi intermediate and cis-Golgi in an ARF1-independent manner; Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic during interphase and centrosomal during mitosis, it localizes to the centrosomes in a microtubule-independent manner and Cytoplasm. Cytoplasmic throughout the cell cycle.
All lanes : Anti-SCYL1 antibody (ab95074) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Detection of SCYL1 by Western Blot of Immunprecipitate.
ab95074 at 1µg/ml staining SCYL1 in HeLa whole cell lysate immunoprecipitated using ab95074 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.