The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
追加情報Is unsuitable for WB.
機能Regulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro. Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region.
配列類似性Belongs to the protein kinase superfamily. Contains 3 HEAT repeats. Contains 1 protein kinase domain.
ドメインThe protein kinase domain is predicted to be catalytically inactive.
細胞内局在Nucleus; Cytoplasm > cytoskeleton > centrosome. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus > cis-Golgi network. Localized to the Endoplasmic reticulum-Golgi intermediate and cis-Golgi in an ARF1-independent manner; Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic during interphase and centrosomal during mitosis, it localizes to the centrosomes in a microtubule-independent manner and Cytoplasm. Cytoplasmic throughout the cell cycle.
Anti SCYL1, at 1 µg/ml, staining SCYL1 in HeLa whole cell lysate immunoprecipitated using ab95073 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 seconds.
Anti-SCYL1 antibody (ab95073) 使用論文
has not yet been referenced specifically in any publications.