製品の概要

  • 製品名Anti-SCYL1 antibody
    SCYL1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to SCYL1
  • アプリケーション適用あり: ICC/IF, Flow Cyt, IHC-Fr, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Recombinant Fragment
  • 免疫原

    Recombinant fragment: DHKSSKSPES DWSSWEAEGS WEQGWQEPSS QEPPPDGTRL ASEYNWGGPE SSDKGDPFAT LSARPSTQDR SRLSWPGRSA RSGGGRWRPN APRGRWPRAP , corresponding to amino acids 373-472 of Human SCYL1 (AAH09967).

  • ポジティブ・コントロール
    • HeLa nuclear lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/50.
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 90 kDa).
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Regulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro.
    Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the protein kinase superfamily.
    Contains 3 HEAT repeats.
    Contains 1 protein kinase domain.
  • ドメインThe protein kinase domain is predicted to be catalytically inactive.
  • 細胞内局在Nucleus; Cytoplasm > cytoskeleton > centrosome. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus > cis-Golgi network. Localized to the Endoplasmic reticulum-Golgi intermediate and cis-Golgi in an ARF1-independent manner; Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic during interphase and centrosomal during mitosis, it localizes to the centrosomes in a microtubule-independent manner and Cytoplasm. Cytoplasmic throughout the cell cycle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Coated vesicle associated kinase of 90 kDa antibody
    • Coated vesicle-associated kinase of 90 kDa antibody
    • CVAK90 antibody
    • GKLP antibody
    • HT019 antibody
    • Likely ortholog of mouse N terminal kinase like protein antibody
    • MGC78454 antibody
    • N terminal kinase like antibody
    • N terminal kinase like protein antibody
    • N-terminal kinase-like protein antibody
    • NKTL antibody
    • NTKL antibody
    • NTKL_HUMAN antibody
    • NTKLBP1 antibody
    • P105 antibody
    • SCY L1 antibody
    • SCY1 like 1 antibody
    • SCY1 like 1 protein 1 antibody
    • SCY1-like protein 1 antibody
    • SCYL 1 antibody
    • Scyl1 antibody
    • SCYL1BP1 antibody
    • TAPK antibody
    • TEIF antibody
    • Telomerase regulation associated protein antibody
    • Telomerase regulation-associated protein antibody
    • Telomerase transcriptional element interacting factor antibody
    • Telomerase transcriptional element-interacting factor antibody
    • Teratoma associated tyrosine kinase antibody
    • Teratoma-associated tyrosine kinase antibody
    • TRAP antibody
    see all

Anti-SCYL1 antibody 画像

  • Anti-SCYL1 antibody (ab76851) at 1/500 dilution + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/5000 dilution

    Predicted band size : 90 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-SCYL1 antibody (ab76851) at 1/500 dilution + HeLa nuclear extract at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

    Predicted band size : 90 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab76851 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76851, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab76851 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76851, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-SCYL1 antibody (ab76851) 使用論文

ab76851 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Whole embryo)
Specification Whole embryo
Fixative Paraformaldehyde
Permeabilization Yes - Ethanol
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 May 01 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"