製品の概要

  • 製品名Anti-SCUBE3 antibody
    SCUBE3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to SCUBE3
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Recombinant Fragment
    交差が予測される動物種: Mouse, Rat, Chicken, Dog, Human, Pig
  • 免疫原

    GST-tagged recombinant fragment, corresponding to amino acids 29-148 of Human SCUBE3 (AAH52263)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight of endogenous protein: 109 kDa.
    Note: this antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 組織特異性Highly expressed in osteoblasts. Expression is low or absent in non-bone tissues.
    • 配列類似性Contains 1 CUB domain.
      Contains 9 EGF-like domains.
    • 翻訳後修飾N-glycosylated.
      May undergo proteolytic cleavage in the C-terminal region.
    • 細胞内局在Secreted. Cell surface.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CEGF3 antibody
      • CUB and EGF containing protein 3 antibody
      • CUB and EGF-like domain-containing protein 3 antibody
      • CUB domain and EGF like repeat containing 3 antibody
      • DKFZp686B09105 antibody
      • DKFZp686B1223 antibody
      • DKFZp686D20108 antibody
      • FLJ34743 antibody
      • OTTHUMP00000016250 antibody
      • SCUB3_HUMAN antibody
      • SCUBE 3 antibody
      • SCUBE3 antibody
      • Signal peptide antibody
      • Signal peptide CUB and EGF like domain containing protein 3 antibody
      • Signal peptide CUB domain EGF like 3 antibody
      see all

    Anti-SCUBE3 antibody 画像

    • Anti-SCUBE3 antibody (ab76798) at 1 µg/ml + immunogen (recombinant GST-tagged fragment from Human protein) at 0.1 µg

      Secondary
      Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

      Predicted band size : 109 kDa
      Observed band size : 39 kDa (why is the actual band size different from the predicted?)

    Anti-SCUBE3 antibody (ab76798) 使用論文

    ab76798 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - whole (heart)
    Loading amount 25 µg
    Specification heart
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Dr. Hua Jiang

    Verified customer

    投稿 Aug 21 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"