製品の概要
-
製品名Anti-SCRIBBLE antibody [EPR4140(2)]
SCRIBBLE 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR4140(2)] to SCRIBBLE
-
由来種Rabbit
-
アプリケーション適用あり: WB, ICC, Flow Cytmore details
適用なし: IHC-P or IP -
種交差性交差種: Human
非交差種: Mouse, Rat -
免疫原
Synthetic peptide, corresponding to residues near the C-terminus of Human SCRIBBLE.
-
ポジティブ･コントロール
- 293T, HeLa and MCF7 cell lysates; permeabilized 293T cells.
-
特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
バッファーpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA -
精製度Tissue culture supernatant
-
ポリ/モノモノクローナル
-
クローン名EPR4140(2)
-
アイソタイプIgG
-
研究分野
関連製品
-
Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab125080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 175 kDa). | |
| ICC | 1/100 - 1/250. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
-
機能Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.
-
組織特異性Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
-
配列類似性Belongs to the LAP (LRR and PDZ) protein family.
Contains 16 LRR (leucine-rich) repeats.
Contains 4 PDZ (DHR) domains. -
翻訳後修飾Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.
-
細胞内局在Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions.
- Information by UniProt
-
参照データベース
- Entrez Gene: 23513 Human
- Omim: 607733 Human
- SwissProt: Q14160 Human
- Unigene: 436329 Human
-
別名
- CRIB 1 antibody
- CRIB1 antibody
- hScrib antibody
see all
画像
-
![Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)](http://a.abcam.com/ps/datasheet/images/125/ab125080/SCRIBBLE-Primary-antibodies-ab125080-1.jpg)
Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 175 kDa -
![Flow Cytometry - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)](http://a.abcam.com/ps/datasheet/images/125/ab125080/SCRIBBLE-Primary-antibodies-ab125080-2.jpg)
Flow Cytometry - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)ab125080 at 1/100, staining SCRIBBLE in permeabilized 293T cells by flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).
プロトコール
参考文献
ab125080 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"