製品の概要
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製品名Anti-SCRIBBLE antibody
SCRIBBLE 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to SCRIBBLE
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, WBmore details
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種交差性交差種: Human
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免疫原
Recombinant fusion protein corresponding to C terminus of human SCRIBBLE (including PDZ3 and 4).
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ポジティブ･コントロール
- Diluted human cell extracts.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: None
Constituents: Whole serum -
精製度Whole antiserum
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab36708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/5000. Predicted molecular weight: 175 kDa. |
ターゲット情報
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機能Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.
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組織特異性Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
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配列類似性Belongs to the LAP (LRR and PDZ) protein family.
Contains 16 LRR (leucine-rich) repeats.
Contains 4 PDZ (DHR) domains. -
翻訳後修飾Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.
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細胞内局在Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions.
- Information by UniProt
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参照データベース
- Entrez Gene: 23513 Human
- Omim: 607733 Human
- SwissProt: Q14160 Human
- Unigene: 436329 Human
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別名
- CRIB 1 antibody
- CRIB1 antibody
- hScrib antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-SCRIBBLE antibody (ab36708)ICC/IF image of ab36708 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36708, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-SCRIBBLE antibody (ab36708)Image courtesy of an anonymous Abreview.All lanes : Anti-SCRIBBLE antibody (ab36708) at 1/5000 dilution
All lanes : Whole cell lysate prepared from prostate cancer cells
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 175 kDa
Observed band size: 175 kDa
Exposure time: 1 minute
No unspecific bands detected.
参考文献
ab36708 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"