製品の概要

  • 製品名Anti-SCP3 antibody [Cor 10G11/7]
    SCP3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Cor 10G11/7] to SCP3
  • 特異性This antibody is an excellent tool for the visualisation of chromosomes in meiotic spreads.
  • アプリケーション適用あり: IHC-P, ELISA, ICC/IF, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Hamster, Zebrafish
  • 免疫原

    Hamster COR1/SCP3 protein (expressed as 6x His fusion in bacteria and purified on a Talon under denaturing conditions).

  • ポジティブ・コントロール
    • WB: Testis (Mouse) Tissue Lysate IHC-P: FFPE human testis tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.

ターゲット情報

  • 機能Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development.
  • 組織特異性Testis-specific.
  • 関連疾患Defects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes.
  • 配列類似性Belongs to the XLR/SYCP3 family.
  • 細胞内局在Nucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed.
  • Information by UniProt
  • 参照データベース
  • 別名
    • choline phosphotransferase 1 antibody
    • chpt1 antibody
    • COR 1 antibody
    • COR1 antibody
    • MGC71888 antibody
    • RNASCP3 antibody
    • SCP 3 antibody
    • SCP-3 antibody
    • SCP3 antibody
    • SPGF4 antibody
    • Sycp 3 antibody
    • Sycp3 antibody
    • SYCP3_HUMAN antibody
    • Synaptonemal complex protein 3 antibody
    see all

Anti-SCP3 antibody [Cor 10G11/7] 画像

  • IHC image of SCP3 staining in a section of formalin-fixed paraffin-embedded human normal testes*. using Mouse on Mouse detection kit, ab127055. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab97672 at 1ug/ml dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

     

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ab97672 staining SCP3 in mouse embryonic day 16.5 female germ cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in Formaldehyde/SDS and permeabilized in 0.05% Tween20 and 0.05% Triton X-100 in 1xPBS prior to blocking in 10% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated goat anti-Mouse IgG polyclonal, diluted 1/400.

    See Abreview

  • All lanes : Anti-SCP3 antibody [Cor 10G11/7] (ab97672) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate
    Lane 2 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Additional bands at : 125 kDa (possible non-specific binding).

    Exposure time : 30 seconds
  • IHC-P image of SCP3 staining with ab97672 on tissue sections from adult mouse testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab97672 (1/200 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal, used at a 1/500 dilution.

    See Abreview

  • ab97672 staining SCP3 in Mouse adult testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a 0.01M citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA) for 1 hour at 22°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

Anti-SCP3 antibody [Cor 10G11/7] (ab97672) 使用論文

This product has been referenced in:
  • Meng X  et al. Genetic Deficiency of Mtdh Gene in Mice Causes Male Infertility via Impaired Spermatogenesis and Alterations in the Expression of Small Non-coding RNAs. J Biol Chem 290:11853-64 (2015). IHC ; Mouse . Read more (PubMed: 25787082) »
  • Nguyen EB  et al. SPACA7 is a novel male germ cell-specific protein localized to the sperm acrosome that is involved in fertilization in mice. Biol Reprod 90:16 (2014). Mouse . Read more (PubMed: 24307706) »

See all 15 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis, adult)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis, adult
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

投稿 Sep 16 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Fish Tissue sections (testis)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: immunosaver 
Specification testis
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 12 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis)
Permeabilization No
Specification Testis
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 22 2016

Application Immunohistochemistry (Frozen sections)
Sample Chicken Cell (Ovaries)
Permeabilization Yes - Triton X-100
Specification Ovaries
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Dr. xiaochuan tang

Verified customer

投稿 Jun 19 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue sections (postnatal 6 day testes)
Specification postnatal 6 day testes
Permeabilization No
Fixative 4% PFA
Username

Dr. Qing Wen

Verified customer

投稿 Apr 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (adult testes)
Specification adult testes
Fixative 4% PFA
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Qing Wen

Verified customer

投稿 Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (adult mouse testis section)
Specification adult mouse testis section
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

煜欽 林

Verified customer

投稿 Sep 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (embryonic day 16.5 female germ cells)
Specification embryonic day 16.5 female germ cells
Fixative Formaldehyde/SDS
Permeabilization Yes - 0.05% Tween20 and 0.05% Triton X-100 in 1xPBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Jason Heaney

Verified customer

投稿 Sep 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"