製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SCP1
  • アプリケーション
    適用あり: IHC-Fr, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide (Mouse) (C terminal).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: Tris citrate/phosphate. pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/750.
IHC-P 1/750.
ICC/IF 1/100 - 1/750.

ターゲット情報

  • 機能
    Major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase.
  • 組織特異性
    Testis.
  • ドメイン
    Consists of an alpha-helical stretch of 700 AA residues, flanked by N- and C-terminal globular domains. The C-terminal domain has DNA-binding capacity.
  • 細胞内局在
    Nucleus. Chromosome. In tripartite segments of synaptonemal complexes, between lateral elements in the nucleus. Found only where the chromosome cores are synapsed. Its N-terminus is found towards the center of the synaptonemal complex while the C-terminus extends well into the lateral domain of the synaptonemal complex.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cancer/testis antigen 8 antibody
    • CT8 antibody
    • HOM TES 14 antibody
    • MGC104417 antibody
    • SCP 1 antibody
    • SCP-1 antibody
    • SCP1 antibody
    • SYCP 1 antibody
    • Sycp1 antibody
    • SYCP1_HUMAN antibody
    • Synaptonemal complex protein 1 antibody
    • Testicular tissue protein Li 191 antibody
    see all

Anti-SCP1 antibody 画像

  • SCP1 labeled in mouse pachytene preparation (red), using ab15090. CDK2 staining, near teleomeres, is also present (green).
  • Paraffin embeded IHC image of Murine SCP1 in mouse ovary using ab15090 at 1:750 dilution and secondary at 1:400.

Anti-SCP1 antibody (ab15090) 使用論文

This product has been referenced in:
  • Takata KI  et al. Analysis of DNA polymerase ? function in meiotic recombination, immunoglobulin class-switching, and DNA damage tolerance. PLoS Genet 13:e1006818 (2017). ICC/IF ; Mouse . Read more (PubMed: 28570559) »
  • Myrick DA  et al. KDM1A/LSD1 regulates the differentiation and maintenance of spermatogonia in mice. PLoS One 12:e0177473 (2017). Read more (PubMed: 28498828) »

See all 37 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (testis)
Permeabilization
Yes - Triton
Specification
testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse testes)
Specification
mouse testes
Fixative
4% PFA
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 07 2013

Thank you for contacting Abcam.

Under our Abpromise we guarantee our products for 6 months from the date of delivery and so it is no longer covered under this guarantee. I will be more than happy to help you troubleshoot your protocol to see ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Spermatocytes)
Specification
Spermatocytes
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1%
Username

Abcam user community

Verified customer

投稿 May 02 2007

I can confirm that ab15090 is not likely to cross react with human as the immunogen shares only 83% homology with the human protein. Unfortunately ab15087 will work in human but does not appear to work in immunohistochemistry on paraffin-embedded sect...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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