製品の概要

  • 製品名Anti-SCP1 antibody
    SCP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SCP1
  • アプリケーション適用あり: ICC/IF, IHC-P, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig, Marmoset (common)
  • 免疫原

    Synthetic peptide (Human) (C terminal).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: Tris citrate/phosphate. pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/300 - 1/750.
IHC-P 1/100.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能Major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase.
  • 組織特異性Testis.
  • ドメインConsists of an alpha-helical stretch of 700 AA residues, flanked by N- and C-terminal globular domains. The C-terminal domain has DNA-binding capacity.
  • 細胞内局在Nucleus. Chromosome. In tripartite segments of synaptonemal complexes, between lateral elements in the nucleus. Found only where the chromosome cores are synapsed. Its N-terminus is found towards the center of the synaptonemal complex while the C-terminus extends well into the lateral domain of the synaptonemal complex.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cancer/testis antigen 8 antibody
    • CT8 antibody
    • HOM TES 14 antibody
    • MGC104417 antibody
    • SCP 1 antibody
    • SCP-1 antibody
    • SCP1 antibody
    • SYCP 1 antibody
    • Sycp1 antibody
    • SYCP1_HUMAN antibody
    • Synaptonemal complex protein 1 antibody
    see all

Anti-SCP1 antibody 画像

  • SCP1 labeled in mouse pachytene preparation (red), using ab15087. CDK2 staining, near teleomeres, is also present (green).
  • IHC-P image of SCP1 staining with ab15087 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab15087 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

Anti-SCP1 antibody (ab15087) 使用論文

This product has been referenced in:
  • Yu-Ching Lin Z  et al. Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development. Dev Biol N/A:N/A (2015). IHC-P ; Marmoset (common) . Read more (PubMed: 25624265) »
  • Baker CL  et al. Multimer Formation Explains Allelic Suppression of PRDM9 Recombination Hotspots. PLoS Genet 11:e1005512 (2015). ICC/IF ; Mouse . Read more (PubMed: 26368021) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (Adult Testis)
Specification Adult Testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

煜欽 林

Verified customer

投稿 Apr 19 2013

Thank you for contacting Abcam.

Under our Abpromise we guarantee our products for 6 months from the date of delivery and so it is no longer covered under this guarantee. I will be more than happy to help you troubleshoot your protocol to see ...

Read More

I can confirm that ab15090 is not likely to cross react with human as the immunogen shares only 83% homology with the human protein. Unfortunately ab15087 will work in human but does not appear to work in immunohistochemistry on paraffin-embedded sect...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cell (spermatocytes)
Specification spermatocytes
Fixative Formaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
Username

Dr. Ahmad Khalil

Verified customer

投稿 Oct 10 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"