Anti-SCNN1B (phospho T615) 抗体 (ab79172)
Key features and details
- Rabbit polyclonal to SCNN1B (phospho T615)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-SCNN1B (phospho T615) antibody
SCNN1B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SCNN1B (phospho T615) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human, African green monkey
交差が予測される動物種: Mouse, Rat -
免疫原
Synthesized phosphopeptide derived from human SCNN1B around the phosphorylation site of threonine 615 (P-G-TP-P-P).
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ポジティブ・コントロール
- Human brain tissue; extracts from HeLa cells; COS7 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79172の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 73 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/500 - 1/1000.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 73 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. -
関連疾患
Pseudohypoaldosteronism 1, autosomal recessive
Liddle syndrome
Bronchiectasis with or without elevated sweat chloride 1 -
配列類似性
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1B subfamily. -
翻訳後修飾
Phosphorylated on serine and threonine residues. -
細胞内局在
Apical cell membrane. Apical membrane of epithelial cells. - Information by UniProt
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参照データベース
- Entrez Gene: 6338 Human
- Entrez Gene: 20277 Mouse
- Entrez Gene: 24767 Rat
- Omim: 600760 Human
- SwissProt: P51168 Human
- SwissProt: Q9WU38 Mouse
- SwissProt: P37090 Rat
- Unigene: 414614 Human
see all -
別名
- Amiloride sensitive sodium channel subunit beta antibody
- Amiloride-sensitive sodium channel subunit beta antibody
- Beta NaCH antibody
see all
画像
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All lanes : Anti-SCNN1B (phospho T615) antibody (ab79172) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from HeLa cells with immunizing phosphopeptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 73 kDa -
Immunohistochemiscal analysis of SCNN1B in paraffin embedded human brain tissue using a 1/50 dilution of ab79172 in the presence (right) or absence (left) of the immunizing phosphopeptide.
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Immunofluorescence analysis of SCNN1B in COS7 cells, using a 1/500 dilution of ab79172, in the presence (right) or absence (left) of the immunizing phosphopeptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab79172 は 1 報の論文で使用されています。
- Shimko MJ et al. Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells. Physiol Rep 2:N/A (2014). WB ; Human . PubMed: 25347857