製品の概要

  • 製品名Anti-SCNN1B antibody
    SCNN1B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SCNN1B
  • 特異性ab28668 recognises Amiloride-sensitive sodium channel beta-subunit (SCNN1B).
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide derived from residues 591-640 (C-terminal) of human SCNN1B.

    .

  • ポジティブ・コントロール
    • Jurkat cell lysate IF/ICC: HepG2 cell line.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab28668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).Can be blocked with SCNN1B peptide (ab177197). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
  • 関連疾患Pseudohypoaldosteronism 1, autosomal recessive
    Liddle syndrome
    Bronchiectasis with or without elevated sweat chloride 1
  • 配列類似性Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1B subfamily.
  • 翻訳後修飾Phosphorylated on serine and threonine residues.
  • 細胞内局在Apical cell membrane. Apical membrane of epithelial cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Amiloride sensitive sodium channel subunit beta antibody
    • Amiloride-sensitive sodium channel subunit beta antibody
    • Beta NaCH antibody
    • Beta-ENaC antibody
    • Beta-NaCH antibody
    • ENaC beta antibody
    • ENaCB antibody
    • Epithelial Na(+) channel subunit beta antibody
    • Epithelial Na+ channel beta subunit antibody
    • Epithelial Na+ channel subunit beta antibody
    • Epithelial sodium channel beta 2 subunit antibody
    • Epithelial sodium channel beta 3 subunit antibody
    • Nonvoltage gated sodium channel 1 beta subunit antibody
    • Nonvoltage gated sodium channel 1 subunit beta antibody
    • Nonvoltage-gated sodium channel 1 subunit beta antibody
    • SCNEB antibody
    • SCNN 1B antibody
    • SCNN1B antibody
    • SCNNB_HUMAN antibody
    • Sodium channel nonvoltage gated 1 beta (Liddle syndrome) antibody
    • Sodium channel nonvoltage gated 1 beta antibody
    see all

Anti-SCNN1B antibody 画像

  • ab28668 stained HepG2 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28668 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-SCNN1B antibody (ab28668) at 0.125 µg/ml + Jurkat cell lysates

    Predicted band size : 70 kDa

Anti-SCNN1B antibody (ab28668) 使用論文

This product has been referenced in:
  • Shimko MJ  et al. Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells. Physiol Rep 2:N/A (2014). WB ; Human . Read more (PubMed: 25347857) »

See 1 Publication for this product

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Thank you for completing the questionnaire and for including the western blotting image.
I am sorry this antibody is giving you trouble. It seems you should be able to detect SCNN1B from MCF7 cells. If you have purchased this antibody within the p...

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