製品の概要
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製品名Anti-SCN3B antibody
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製品の詳細Rabbit polyclonal to SCN3B
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由来種Rabbit
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アプリケーション適用あり: WBmore details
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種交差性交差種: Rat, Human
交差が予測される動物種: Mouse, Cow
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免疫原
A region within synthetic peptide: RPEGGKDFLI YEYRNGHQEV ESPFQGRLQW NGSKDLQDVS ITVLNVTLND, corresponding to amino acids 65-114 of Human SCN3B
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ポジティブ･コントロール
- Fetal brain lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab48552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.5 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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機能Modulates channel gating kinetics. Causes unique persistent sodium currents. Inactivates the sodium channel opening more slowly than the subunit beta-1. Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons.
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関連疾患Defects in SCN3B are the cause of Brugada syndrome type 7 (BRGDA7) [MIM:613120]. A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset.
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配列類似性Belongs to the sodium channel auxiliary subunit SCN3B (TC 8.A.17) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 540925 Cow
- Entrez Gene: 55800 Human
- Entrez Gene: 235281 Mouse
- Entrez Gene: 245956 Rat
- Omim: 608214 Human
- SwissProt: Q2KI11 Cow
- SwissProt: Q9NY72 Human
- SwissProt: Q8BHK2 Mouse
see all -
別名
- 1110001K16Rik antibody
- 4833414B02Rik antibody
- HSA243396 antibody
see all
画像
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Western blot - Anti-SCN3B antibody (ab48552)Anti-SCN3B antibody (ab48552) at 0.5 µg/ml + fetal brain lysate at 10 µg with 5% skimmed milk in PBS buffer
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Gel concentration: 15%
プロトコール
参考文献
This product has been referenced in:
- Shy D et al. PDZ domain-binding motif regulates cardiomyocyte compartment-specific NaV1.5 channel expression and function. Circulation 130:147-60 (2014). Read more (PubMed: 24895455) »
- Ho C et al. Differential expression of sodium channel ß subunits in dorsal root ganglion sensory neurons. J Biol Chem : (2012). WB ; Rat . Read more (PubMed: 22408255) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"