製品の概要

  • 製品名Anti-Scn1a antibody
    Scn1a 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Scn1a
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: IHC-FoFr
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Zebrafish
  • 免疫原

    Synthetic peptide:

    EEQKKYYNAMKKLGSKKP

    , corresponding to C terminal amino acids 1491-1508 of Rat Scn1a

  • ポジティブ・コントロール
    • Brain
  • 特記事項Ab with sodium azide is stable for 24 months when stored at 2-8 °C.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab24820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 220 kDa.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for IHC-FoFr.
  • ターゲット情報

    • 機能Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
    • 関連疾患Defects in SCN1A are the cause of generalized epilepsy with febrile seizures plus type 2 (GEFS+2) [MIM:604233]. Generalized epilepsy with febrile seizures-plus refers to a rare autosomal dominant, familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity.
      Defects in SCN1A are a cause of severe myoclonic epilepsy in infancy (SMEI) [MIM:607208]; also called Dravet syndrome. SMEI is a rare disorder characterized by generalized tonic, clonic, and tonic-clonic seizures that are initially induced by fever and begin during the first year of life. Later, patients also manifest other seizure types, including absence, myoclonic, and simple and complex partial seizures. Psychomotor development delay is observed around the second year of life. SMEI is considered to be the most severe phenotype within the spectrum of generalized epilepsies with febrile seizures-plus.
      Defects in SCN1A are a cause of intractable childhood epilepsy with generalized tonic-clonic seizures (ICEGTC) [MIM:607208]. ICEGTC is a disorder characterized by generalized tonic-clonic seizures beginning usually in infancy and induced by fever. Seizures are associated with subsequent mental decline, as well as ataxia or hypotonia. ICEGTC is similar to SMEI, except for the absence of myoclonic seizures.
      Defects in SCN1A are the cause of migraine familial hemiplegic type 3 (FHM3) [MIM:609634]. FHM3 is an autosomal dominant severe subtype of migraine with aura characterized by some degree of hemiparesis during the attacks. The episodes are associated with variable features of nausea, vomiting, photophobia, and phonophobia. Age at onset ranges from 6 to 15 years. FHM is occasionally associated with other neurologic symptoms such as cerebellar ataxia or epileptic seizures. A unique eye phenotype of elicited repetitive daily blindness has also been reported to be cosegregating with FHM in a single Swiss family.
      Defects in SCN1A are the cause of familial febrile convulsions type 3A (FEB3A) [MIM:604403]; also known as familial febrile seizures 3. Febrile convulsions are seizures associated with febrile episodes in childhood without any evidence of intracranial infection or defined pathologic or traumatic cause. It is a common condition, affecting 2-5% of children aged 3 months to 5 years. The majority are simple febrile seizures (generally defined as generalized onset, single seizures with a duration of less than 30 minutes). Complex febrile seizures are characterized by focal onset, duration greater than 30 minutes, and/or more than one seizure in a 24 hour period. The likelihood of developing epilepsy following simple febrile seizures is low. Complex febrile seizures are associated with a moderately increased incidence of epilepsy.
    • 配列類似性Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.1/SCN1A subfamily.
      Contains 1 IQ domain.
    • ドメインThe sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • brain sodium channel type I antibody
      • EIEE6 antibody
      • FEB3 antibody
      • FEB3A antibody
      • FHM3 antibody
      • GEFS+2 antibody
      • GEFSP2 antibody
      • HBSC I antibody
      • HBSCI antibody
      • NAC1 antibody
      • Nav 1.1 antibody
      • RBI antibody
      • SCN1 antibody
      • Scn1a antibody
      • SCN1A_HUMAN antibody
      • SMEI antibody
      • Sodium channel protein brain I alpha subunit antibody
      • Sodium channel protein brain I subunit alpha antibody
      • Sodium channel protein type 1 subunit alpha antibody
      • Sodium channel protein type I subunit alpha antibody
      • Sodium channel voltage gated type 1 alpha subunit antibody
      • Sodium channel voltage gated type I alpha polypeptide antibody
      • Voltage-gated sodium channel subunit alpha Nav1.1 antibody
      see all

    Anti-Scn1a antibody 画像

    • Staining of human cerebellum with anti-sodium channel antibody. The tissue was boiled in 10mM citrate buffer, pH 6.0 for 10 mins followed by cooling at room temparture for 20 mins. The working dilution for the antibody is 1:200 for 30 min at room temparture.
    • ICC/IF image of ab24820 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24820, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-Scn1a antibody (ab24820) 使用論文

    ab24820 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. We have all the data and Abreviews (submitted by customers) on our online datasheet. Please see: http://www.abcam.com/scn1a-antibody-ab24820.html The ICC/IF information is as follows: ICC/IF image of ab248...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Dorsal Root Ganglion)
    Specification Dorsal Root Ganglion
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Dr. Karine Thibault

    Verified customer

    投稿 Nov 09 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Whole brain homogenate)
    Loading amount 10 µg
    Specification Whole brain homogenate
    Gel Running Conditions Reduced Denaturing (Bis-tris 4-12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Brian Hitt

    Verified customer

    投稿 May 11 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - other (Hippocampal Synaptosome Preparation)
    Loading amount 4 µg
    Specification Hippocampal Synaptosome Preparation
    Gel Running Conditions Reduced Denaturing (4-12% Bis Tris)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 25 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Brain)
    Loading amount 10 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    投稿 Dec 16 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (Brain)
    Loading amount 20 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 07 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Brain)
    Specification Brain
    Fixative formalin
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6.0
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Vlady Pavlova

    Verified customer

    投稿 Dec 11 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"