製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SCML2
  • アプリケーション
    適用あり: IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1-50 of Human SCML2 (Q9UQR0).

  • ポジティブ・コントロール
    • HeLa cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2-10 µg/mg of lysate.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development.
    • 組織特異性
      Highly expressed in placenta, thymus and testis. Detected at lower levels in brain, liver, skeletal muscle, pancreas and ovary.
    • 配列類似性
      Belongs to the SCM family.
      Contains 2 MBT repeats.
      Contains 1 SAM (sterile alpha motif) domain.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • SCML2 antibody
      • SCML2_HUMAN antibody
      • sex comb on midleg like 2 (Drosophila) antibody
      • Sex comb on midleg like 2 antibody
      • Sex comb on midleg like protein 2 antibody
      • Sex comb on midleg, Drosophila, homolog-like 2 antibody
      • Sex comb on midleg-like protein 2 antibody
      see all

    画像

    • Immunoprecipitation of SCML2 (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-SCML2 antibody (ab115157); Lane 2-Immunoprecipitation with an anti-SCML2 antibody that recognizes an downstream epitope (ab115160) ; Lane 3- Immunoprecipitation with IgG control. Anti-SCML2 antibody from lane 2 (ab1151600) was used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 3o sec.

    プロトコール

    参考文献

    ab115157 has not yet been referenced specifically in any publications.

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