製品の概要

  • 製品名Anti-SCML1 antibody
    SCML1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SCML1
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Pig, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (ESYSPTLPVS RRENNSPSNL PRPSFCMEEY QRAELEEDPI LSRTPSPVHP) of Human SCML1 (NP_006737).

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab83665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

ターゲット情報

  • 機能Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. May be involved in spermatogenesis during sexual maturation.
  • 組織特異性Ubiquitous. Expressed in fetal and adult tissues.
  • 配列類似性Belongs to the SCM family.
    Contains 1 SAM (sterile alpha motif) domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Gm1792 antibody
    • Gm47 antibody
    • OTTHUMP00000022996 antibody
    • OTTHUMP00000025692 antibody
    • RP3-389A20.1 antibody
    • SCML1 antibody
    • SCML1_HUMAN antibody
    • Sex comb on midleg (Drosophila) like 1 antibody
    • Sex comb on midleg like 1 (Drosophila) antibody
    • Sex comb on midleg like 1 antibody
    • Sex comb on midleg like protein 1 antibody
    • Sex comb on midleg-like protein 1 antibody
    • Vsex comb on midleg like 1 antibody
    see all

Anti-SCML1 antibody 画像

  • Anti-SCML1 antibody (ab83665) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling SCML1 with ab83665 at 5.0µg/ml.

Anti-SCML1 antibody (ab83665) 使用論文

ab83665 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"