製品の概要

  • 製品名Anti-SCMH1 antibody
    SCMH1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to SCMH1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Cynomolgus Monkey
  • 免疫原

    Recombinant fragment: EKLCHNLRSD NLFGNQPFTQ THLSLTAIEY SHSHDRYLPG ETFVLGNSLA RSLEPHSDSM DSASNPTNLV STSQRHRPLL SSCGLPPSTA SAVRRLCSR, corresponding to amino acids 404-502 of Human SCMH1 (NP_036368) with proprietory tag.

  • ポジティブ・コントロール
    • HepG2 cell lysate. Recombinant tagged human SCMH1 fragment.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab77648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 73 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged SCMH1 is approximately 30ng/ml when used as a capture antibody.

ターゲット情報

  • 機能Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • 組織特異性Strongly expressed in heart, muscle and pancreas. Weakly expressed in brain, placenta, lung, liver and kidney.
  • 配列類似性Belongs to the SCM family.
    Contains 2 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Polycomb protein SCMH1 antibody
    • SCMH 1 antibody
    • SCMH1 antibody
    • SCMH1_HUMAN antibody
    • Scml 3 antibody
    • Scml3 antibody
    • Sex comb on midleg 1 antibody
    • Sex comb on midleg homolog 1 antibody
    see all

Anti-SCMH1 antibody 画像

  • Anti-SCMH1 antibody (ab77648) at 1 µg/ml + Recombinant tagged human SCMH1 fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 73 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-SCMH1 antibody (ab77648) at 1 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

Anti-SCMH1 antibody (ab77648) 使用論文

ab77648 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"