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Synthetic peptide corresponding to Human Sclerostin aa 12-42 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: 50964
Our Abpromise guarantee covers the use of ab63097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).|
|IHC-P||1/10 - 1/50.|
ab63097 staining Sclerostin in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. An undiluted Biotin-conjugated Goat polyclonal was used as the secondary antibody.
Immunohistochemical analysis of PFA-fixed paraffin-embedded rat femural tissue, labelling sclerostin with ab63097 at a dilution of 1/50 incubated for 13 hours at 4°C in 1% BSA in TBS. Heat mediated antigen retrival was performed via Tris-EDTA pH 9.0. Blocking was via ab93695 ABC kit incubated at 1% for 20 minutes at room temperature. A sexondary was not used, but ab93695 detection kit was used for signal amplification.
This image is courtesy of an Abreview submitted by Helder Fonseca
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Sclerostin with ab63097. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.