製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SCIN
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 281 and 555 of Human SCIN.

  • ポジティブ・コントロール
    • HepG2 whole cell lysate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 80 kDa.

ターゲット情報

  • 機能
    Ca(2+)-dependent actin filament-severing protein that is presumed to have a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. In vitro, also has barbed end capping and nucleating activities in the presence of Ca(2+). Regulates chondrocyte proliferation and differentiation. MAP kinases p38 and ERK1/2 mediate the adseverin-induced accelerated differentiation of non-hypertrophic chondrocytes.
  • 配列類似性
    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
  • 細胞内局在
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adseverin antibody
    • ADSV_HUMAN antibody
    • KIAA1905 antibody
    • Scin antibody
    • Scinderin antibody
    see all

画像

  • Anti-SCIN antibody (ab96105) at 1/1000 dilution + HepG2 cell lysate at 30 µg

    Predicted band size : 80 kDa

プロトコール

参考文献

ab96105 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bos taurus Cell lysate - whole cell (Chondrocytes)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
10 µg
Specification
Chondrocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Byron Chan

Verified customer

投稿 Nov 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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