製品の概要
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製品名Anti-SCD1 antibody [CD.E10]
SCD1 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [CD.E10] to SCD1
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由来種Mouse
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アプリケーション適用あり: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WB, IP, ELISAmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Recombinant full length protein (Human).
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ポジティブ･コントロール
- NIH 3T3 cells
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特記事項SCD1 is known to undergo post-translational modifications and the sizes differ in different cell lines so the observed band size can be different than predicted band size. As positive control we recommend using SCD1 over-expressed 293 transfected cell lysates for western blot.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
精製度Ascites
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特記事項（精製）Protein A affinity chromatography
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ポリ/モノモノクローナル
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クローン名CD.E10
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アイソタイプIgG2b
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab19862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 4 µg/ml. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 20530718 | |
| Flow Cyt | Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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| WB | 1/1000. Predicted molecular weight: 41.5 kDa. PubMed: 18697866 | |
| IP | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. |
ターゲット情報
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機能Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA.
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配列類似性Belongs to the fatty acid desaturase family.
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ドメインThe histidine box domains may contain the active site and/or be involved in metal ion binding.
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細胞内局在Endoplasmic reticulum membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 6319 Human
- Entrez Gene: 20249 Mouse
- Entrez Gene: 83792 Rat
- Omim: 604031 Human
- SwissProt: O00767 Human
- SwissProt: P13516 Mouse
- SwissProt: P07308 Rat
- Unigene: 558396 Human
see all -
別名
- ACOD_HUMAN antibody
- Acyl-CoA desaturase antibody
- Delta(9)-desaturase antibody
see all
画像
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![Western blot - Anti-SCD1 antibody [CD.E10] (ab19862)](http://a.abcam.com/ps/products/19/ab19862/Images/ab19862_1.jpg)
Western blot - Anti-SCD1 antibody [CD.E10] (ab19862)Western blot using ab19862 on 293 cells. Western blot using ab19862 on 293 cells. -
![Immunoprecipitation - Anti-SCD1 antibody [CD.E10] (ab19862)](http://a.abcam.com/ps/products/19/ab19862/Images/SCD1-Primary-antibodies-ab19862-7.jpg)
Immunoprecipitation - Anti-SCD1 antibody [CD.E10] (ab19862)SCD was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Mouse monoclonal to SCD and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab19862.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 32kDa; SCD. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [CD.E10] (ab19862)](http://a.abcam.com/ps/products/19/ab19862/Images/SCD-Primary-antibodies-ab19862-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [CD.E10] (ab19862)ab19862 staining SCD in human skin.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
![Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [CD.E10] (ab19862)](http://a.abcam.com/ps/products/19/ab19862/Images/SCD-Primary-antibodies-ab19862-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [CD.E10] (ab19862)ICC/IF image of ab19862 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19862, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Flow Cytometry - Anti-SCD1 antibody [CD.E10] (ab19862)](http://a.abcam.com/ps/products/19/ab19862/Images/SCD-Primary-antibodies-ab19862-4.jpg)
Flow Cytometry - Anti-SCD1 antibody [CD.E10] (ab19862)Overlay histogram showing HepG2 cells stained with ab19862 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19862, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
プロトコール
参考文献
This product has been referenced in:
- Wu G et al. Menin enhances c-Myc-mediated transcription to promote cancer progression. Nat Commun 8:15278 (2017). WB ; Human . Read more (PubMed: 28474697) »
- Paiardi C et al. The Stearoyl-CoA Desaturase-1 (Desat1) in Drosophila cooperated with Myc to Induce Autophagy and Growth, a Potential New Link to Tumor Survival. Genes (Basel) 8:N/A (2017). Read more (PubMed: 28452935) »
レビューと Q&A
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