製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SCD1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Goat, Guinea pig, Cat, Dog
  • 免疫原

    A region within a synthetic peptide: HVGWLLVRKH PAVKEKGSTL DLSDLEAEKL VMFQRRYYKP GLLMMCFILP, corresponding to internal sequence amino acids 181-230 of Human SCD

  • ポジティブ・コントロール
    • Fetal liver lysate Human liver tissue slide

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab23331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA.
  • 配列類似性
    Belongs to the fatty acid desaturase family.
  • ドメイン
    The histidine box domains may contain the active site and/or be involved in metal ion binding.
  • 細胞内局在
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACOD_HUMAN antibody
    • Acyl-CoA desaturase antibody
    • Delta(9)-desaturase antibody
    • Delta-9 desaturase antibody
    • Delta-9-Desaturase antibody
    • FADS5 antibody
    • Fatty acid desaturase antibody
    • PRO0998 antibody
    • SCD 1 antibody
    • SCD antibody
    • SCD1 antibody
    • Stearoyl-CoA desaturase antibody
    see all

画像

  • Anti-SCD1 antibody (ab23331) at 1 µg/ml + Fetal liver lysate

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000

    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SCD1 with ab23331 at a concentration of 4-8µg/ml. Cells with positive staining are marked by arrows. Magnification: X400.

参考文献

This product has been referenced in:

See all 2 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (PAGE 12%)
Sample
Cow Cell lysate - whole cell (bovine fetal liver lysate)
Specification
bovine fetal liver lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jun 09 2014

Thank you for contacting us and for your interest in our products.

As far as we are aware, ab23331 has never been tested in ELISA so we do not know whether or not it is suitable for this application. All tested applications are specified on ...

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Thank you for contacting us.

According to SwissProt, the predicted MW of the SCD human protein is 41.5 kDa.

http://www.uniprot.org/uniprot/O00767

We made a note on the datasheet to point out that the band observed at 37 k...

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DISCOUNT CODE: ***
Expiration date: September 11th, 2012

I am very pleased to hear you would like to accept our offer and test ab23331 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this of...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (liver microsomes)
Loading amount
10 µg
Specification
liver microsomes
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
Username

Dr. Colin Shearn

Verified customer

投稿 Jan 27 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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The peptide used was derived from that region - residues 184-238 of Human SCD. The antibody has not been tested against the SCD2 or SCD5 isoform. Please contact us again if you have any additional questions.

I was informed that the following method was used with ab23331 immunostaining of human liver sections: 1. Deparaffinize sections in 3 changes of xylene, 5 minutes each 2. Hydrate in 2 changes of 100% ethanol for 3 minutes each, 95% and 80% ethan...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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